Mueller U W, Hawes C S, Jones W R
J Immunol Methods. 1986 Mar 13;87(2):193-6. doi: 10.1016/0022-1759(86)90530-2.
Incomplete Freund's adjuvant was used as a priming agent prior to the injection of hybridoma cells in mice to expand monoclonal antibody production. Two hybridoma cell lines, FDO28B (IgG1) and FDO31C (IgM), which produce monoclonal antibodies reactive towards human placenta, were used. Monoclonal antibody was detected in the ascites fluids by agarose gel electrophoresis. It was found that the time interval between adjuvant priming and cell injection could be reduced to 1 day, allowing collection of ascites fluid containing monoclonal antibody within 2 weeks of priming. In addition, as few as 1 X 10(5) hybridoma cells were needed to collect approximately 5-7 ml of ascites fluid containing antibody detectable by gel electrophoresis. Thus priming with incomplete Freund's adjuvant enables production of large amounts of monoclonal antibody in a short time using a low number of hybridoma cells.
在向小鼠注射杂交瘤细胞以扩大单克隆抗体产量之前，使用不完全弗氏佐剂作为启动剂。使用了两种产生对人胎盘有反应的单克隆抗体的杂交瘤细胞系，FDO28B（IgG1）和FDO31C（IgM）。通过琼脂糖凝胶电泳在腹水中检测到单克隆抗体。发现佐剂启动与细胞注射之间的时间间隔可缩短至1天，从而在启动后2周内收集到含有单克隆抗体的腹水。此外，只需1×10⁵个杂交瘤细胞就能收集到约5 - 7毫升可通过凝胶电泳检测到抗体的腹水。因此，用不完全弗氏佐剂启动能够在短时间内使用少量杂交瘤细胞产生大量单克隆抗体。
