一种测量 cGMP 信号和 cGMP 介导的血管舒张的入门指南。
A primer for measuring cGMP signaling and cGMP-mediated vascular relaxation.
机构信息
Heart, Lung, Blood and Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA, USA; Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA, USA; Center for Microvascular Research, University of Pittsburgh, Pittsburgh, PA, USA.
Department of Pharmacology, Physiology and Neuroscience, Rutgers New Jersey Medical School, 185 South Orange Ave., MSBI655, 07103, Newark, NJ, USA.
出版信息
Nitric Oxide. 2021 Dec 1;117:40-45. doi: 10.1016/j.niox.2021.09.008. Epub 2021 Sep 30.
Soluble guanylyl cyclase (sGC, also called GC1) is the main receptor for nitric oxide (NO) that catalyzes the production of the second messenger molecule, 3'5' cyclic guanosine monophosphate (cGMP) leading to vasorelaxation, and inhibition of leukocyte recruitment and platelet aggregation. Enhancing cGMP levels, through sGC agonism or inhibition of cGMP breakdown via phosphodiesterase inhibition, has yielded FDA approval for several cGMP modifier therapies for treatment of cardiovascular and pulmonary diseases. While basic research continues to improve our understanding of cGMP signaling and as new therapies evolve to elevate cGMP levels, we provide a short methodological primer for measuring cGMP and cGMP-mediated vascular relaxation for investigators.
可溶性鸟苷酸环化酶（sGC，也称为 GC1）是一氧化氮（NO）的主要受体，可催化第二信使分子 3'5' 环鸟苷酸单磷酸（cGMP）的产生，导致血管舒张，并抑制白细胞募集和血小板聚集。通过 sGC 激动剂或抑制磷酸二酯酶抑制 cGMP 的分解来提高 cGMP 水平，已经为几种 cGMP 修饰疗法获得了 FDA 批准，用于治疗心血管和肺部疾病。虽然基础研究继续提高我们对 cGMP 信号转导的理解，并且随着新疗法的出现来提高 cGMP 水平，但我们为研究人员提供了一个简短的方法学入门，用于测量 cGMP 和 cGMP 介导的血管舒张。
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