College of Veterinary Medicine, Jilin Agricultural University, Changchun, China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China; Key Laboratory of animal production and product quality safety of Ministry of Education, Jilin Agricultural University, Changchun, China.
College of Veterinary Medicine, Jilin Agricultural University, Changchun, China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China; Key Laboratory of animal production and product quality safety of Ministry of Education, Jilin Agricultural University, Changchun, China.
Int Immunopharmacol. 2021 Dec;101(Pt A):108184. doi: 10.1016/j.intimp.2021.108184. Epub 2021 Sep 30.
Trichinellosis is a foodborne zoonosis caused by Trichinella spiralis (T. spiralis) that not only causes considerable economic losses for the global pig breeding and food industries, but also seriously threats the health of human. Therefore, it is very necessary to develop an effective vaccine to prevent trichinellosis. In this study, the invasive Lactobacillus plantarum (L. plantarum) expressing fibronectin-binding protein A (FnBPA) was served as a live bacterial vector to deliver DNA to the host to produce a novel oral DNA vaccine. Co-expressing T. spiralis SS1 and murine interleukin-4 (mIL-4) of DNA vaccine were constructed and subsequently delivered to intestinal epithelial cells via invasive L. plantarum. At 10 days after the third immunization, the experimental mice were challenged with 350 T. spiralis infective larvae. The results found that the mice orally vaccinated with invasive L. plantarum harboring pValac-SS1/pSIP409-FnBPA not only stimulated the production of anti-SS1-specific IgG, Th1/Th2 cell cytokines, and secreted(s) IgA but also decreased worm burden and intestinal damage. However, the mice inoculated with invasive L. plantarum co-expressing SS1 and mIL-4 (pValac-SS1-IL-4/pSIP409-FnBPA) induced the highest protective immune response against T. spiralis infection. The DNA vaccine delivered by invasive L. plantarum provides a novel idea for the prevention of T. spiralis infection.
旋毛虫病是一种由旋毛虫（T. spiralis）引起的食源性人畜共患病，不仅给全球养猪和食品工业造成了巨大的经济损失，还严重威胁人类健康。因此，开发有效的疫苗来预防旋毛虫病非常必要。本研究以侵袭性植物乳杆菌（L. plantarum）表达纤维连接蛋白结合蛋白 A（FnBPA）作为活细菌载体，将 DNA 递送至宿主产生新型口服 DNA 疫苗。构建了共表达旋毛虫 SS1 和鼠白细胞介素-4（mIL-4）的 DNA 疫苗，并通过侵袭性植物乳杆菌递送至肠上皮细胞。在第三次免疫后 10 天，用 350 条旋毛虫感染性幼虫对实验小鼠进行攻毒。结果发现，口服接种携带 pValac-SS1/pSIP409-FnBPA 的侵袭性植物乳杆菌的小鼠不仅刺激了抗 SS1 特异性 IgG、Th1/Th2 细胞细胞因子和分泌型 IgA 的产生，而且还降低了虫荷和肠道损伤。然而，接种共表达 SS1 和 mIL-4 的侵袭性植物乳杆菌（pValac-SS1-IL-4/pSIP409-FnBPA）的小鼠诱导了针对旋毛虫感染的最高保护免疫应答。侵袭性植物乳杆菌递送的 DNA 疫苗为预防旋毛虫感染提供了新的思路。
