School of Public Health, Key Lab of Public Health Safety of the Ministry of Education and NHC Key Lab of Health Technology Assessment, Fudan University, Shanghai 200032, China.
Department of Environmental Health, Harvard TH Chan School of Public Health, Boston, MA, USA.
J Hazard Mater. 2022 Feb 15;424(Pt A):127359. doi: 10.1016/j.jhazmat.2021.127359. Epub 2021 Sep 26.
The biological mechanism of adverse health outcomes related to exposure to traffic-related air pollution (TRAP) needs elucidation. We conducted a randomized, crossover trial among healthy young students in Shanghai, China. Participants wore earplugs and were randomly assigned to a 4-hour walking treatment either along a traffic-polluted road or through a traffic-free park. We conducted untargeted analyses of plasma exosome transcriptomics, serum mass spectrometry-based proteomics, and serum metabolomics to evaluate changes in genome-wide transcription, protein, and metabolite profiles in 35 randomly selected participants. Mean personal exposure levels of ultrafine particles, black carbon, nitrogen dioxide, and carbon monoxide in the road were 2-3 times higher than that in the park. We observed 3449 exosome mRNAs, 58 serum proteins, and 128 serum metabolites that were significantly associated with TRAP. The multi-omics analysis showed dozens of regulatory pathways altered in response to TRAP, such as inflammation, oxidative stress, coagulation, endothelin-1 signaling, and renin-angiotensin signaling. We found that several novel pathways activated in response to TRAP exposure: growth hormone signaling, adrenomedullin signaling, and arachidonic acid metabolism. Our study served as a demonstration and proof of concept on the evidence that associated TRAP exposure with global molecular changes based on the multi-omics level.
交通相关空气污染（TRAP）暴露相关不良健康后果的生物学机制尚需阐明。我们在中国上海的健康年轻学生中进行了一项随机、交叉试验。参与者佩戴耳塞，并随机分配进行 4 小时步行治疗，要么沿着交通污染的道路，要么穿过交通免费的公园。我们对血浆外泌体转录组学、基于血清质谱的蛋白质组学和血清代谢组学进行了非靶向分析，以评估 35 名随机选择的参与者的全基因组转录、蛋白质和代谢物图谱的变化。道路上超细颗粒、黑碳、二氧化氮和一氧化碳的个人平均暴露水平比公园高 2-3 倍。我们观察到与 TRAP 相关的 3449 个外泌体 mRNA、58 种血清蛋白和 128 种血清代谢物。多组学分析显示，数十条调节途径因 TRAP 而改变，如炎症、氧化应激、凝血、内皮素-1 信号和肾素-血管紧张素信号。我们发现了一些新的途径在暴露于 TRAP 后被激活：生长激素信号、肾上腺髓质素信号和花生四烯酸代谢。我们的研究证明并证实了基于多组学水平的 TRAP 暴露与全球分子变化相关的证据。
