Carol and Richard Yu Peritoneal Dialysis Research Centre, Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
Nephrol Dial Transplant. 2022 Sep 22;37(10):1935-1943. doi: 10.1093/ndt/gfab287.
There are limited data on the association of adipose microRNA expression with body composition and adverse clinical outcomes in patients with advanced chronic kidney disease (CKD). We aimed to evaluate the association of adipose miR-130b and miR-17-5p expressions with body composition, functional state, cardiovascular outcome and mortality in incident dialysis patients.
We performed a single-center prospective cohort study. Patients who were planned for peritoneal dialysis were recruited. miR-130b and miR-17-5p expressions were measured from subcutaneous and pre-peritoneal fat tissue obtained during peritoneal dialysis catheter insertion. Body composition and physical function were assessed by bioimpedance spectroscopy and Clinical Frailty Scale. Primary outcome was 2-year survival. Secondary outcomes were 2-year technique survival and major adverse cardiovascular event (MACE) rate.
Adipose expression of miR-130b and miR-17-5p correlated with parameters of muscle mass including intracellular water (miR-130b: r = 0.191, P = 0.02; miR-17-5p: r = 0.211, P = 0.013) and lean tissue mass (miR-17-5p: r = 0.176, P = 0.04; miR-17-5p: r = 0.176, P = 0.004). miR-130b expression predicted frailty significantly (P = 0.017). Adipose miR-17-5p expression predicted 2-year all-cause survival (P = 0.020) and technique survival (P = 0.036), while miR-130b expression predicted incidence of MACE (P = 0.015).
Adipose miR-130b and miR-17-5p expressions correlated with body composition parameters, frailty, and predicted cardiovascular events and mortality in advanced CKD patients.
关于脂肪组织微 RNA 表达与晚期慢性肾脏病（CKD）患者的身体成分和不良临床结局的关系，数据有限。本研究旨在评估脂肪组织 miR-130b 和 miR-17-5p 表达与新进入透析的患者的身体成分、功能状态、心血管结局和死亡率之间的关系。
我们进行了一项单中心前瞻性队列研究。招募了计划进行腹膜透析的患者。在腹膜透析导管插入期间，从皮下和腹膜前脂肪组织中测量了 miR-130b 和 miR-17-5p 的表达。通过生物阻抗谱和临床虚弱量表评估身体成分和身体功能。主要结局为 2 年生存率。次要结局为 2 年技术生存率和主要不良心血管事件（MACE）发生率。
miR-130b 和 miR-17-5p 的脂肪组织表达与肌肉质量参数相关，包括细胞内水（miR-130b：r=0.191，P=0.02；miR-17-5p：r=0.211，P=0.013）和瘦组织质量（miR-17-5p：r=0.176，P=0.04；miR-17-5p：r=0.176，P=0.004）。miR-130b 表达显著预测虚弱（P=0.017）。脂肪组织 miR-17-5p 表达预测 2 年全因生存率（P=0.020）和技术生存率（P=0.036），而 miR-130b 表达预测 MACE 发生率（P=0.015）。
脂肪组织 miR-130b 和 miR-17-5p 表达与身体成分参数、虚弱程度以及预测心血管事件和晚期 CKD 患者的死亡率相关。
