Wang Xin, Du Gengan, Chen Hong, Zeng Xuejun, Liu Bin, Guo Chunfeng, Sheng Qinglin, Yuan Yahong, Yue Tianli
College of Food Science and Engineering, Northwest A&F University, Xianyang, China.
Laboratory of Quality & Safety Risk Assessment for Agro-Products (Yangling), Ministry of Agriculture, Xianyang, China.
Front Microbiol. 2021 Sep 16;12:705681. doi: 10.3389/fmicb.2021.705681. eCollection 2021.
Fuzhuan brick tea (FBT) is a unique post-fermented tea product, naturally co-fermented by microorganisms, and has gained global popularity due to its potential health benefits for humans. Considerable efforts have been made toward elucidating the microbial diversity within FBT, but an understanding of the underlying FBT community interactions and functions remains poorly studied. Consequently, the microbial communities of two types of FBT, originating from Hunan and Shaanxi provinces, were investigated using comparative shotgun metagenomic sequencing and functional annotations. Metagenomic analysis indicated that two communities shared similar taxonomic and functional attributes. Two samples shared 486 genera, in which contributed most to the abundant functions within the two samples. The carbohydrate active enzyme functions of the communities primarily comprised GH (32.92%), GT (26.8%), CEs (20.43%), and AAs (18.04%). Furthermore, the overall metabolic pathways encoded by the metagenomes were largely associated with carbohydrate and amino acid metabolism, with nine metabolic pathways that were differential between two groups including penicillin and cephalosporin biosynthesis. Significantly, a total of 35 potential probiotics were inferred, with being the most abundant inferred probiotic (80%) within the FBT communities. This study provides new insights into FBT microbial communities on their potential functions and roles in FBT characteristics.
茯砖茶（FBT）是一种独特的后发酵茶产品，由微生物自然共同发酵而成，因其对人体潜在的健康益处而在全球受到欢迎。人们在阐明茯砖茶中的微生物多样性方面付出了巨大努力，但对茯砖茶群落潜在的相互作用和功能的了解仍然很少。因此，利用比较鸟枪法宏基因组测序和功能注释对来自湖南和陕西的两种茯砖茶的微生物群落进行了研究。宏基因组分析表明，两个群落具有相似的分类学和功能属性。两个样本共有486个属，其中对两个样本中的丰富功能贡献最大。群落的碳水化合物活性酶功能主要包括糖苷水解酶（GH，32.92%）、糖基转移酶（GT，26.8%）、碳水化合物酯酶（CEs，20.43%）和辅助活性酶（AAs，18.04%）。此外，宏基因组编码的整体代谢途径在很大程度上与碳水化合物和氨基酸代谢相关，两组之间有九条不同的代谢途径，包括青霉素和头孢菌素的生物合成。值得注意的是，总共推断出35种潜在益生菌，其中[具体菌种]是茯砖茶群落中最丰富的推断益生菌（80%）。本研究为茯砖茶微生物群落的潜在功能及其在茯砖茶特性中的作用提供了新的见解。
