Xiang Meichun, Chu Jun, Cai Wenjiao, Ma Haikun, Zhu Weijing, Zhang Xiaoling, Ren Jinwei, Xiao Lizheng, Liu Dongbo, Liu Xingzhong
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
University of Chinese Academy of Sciences, Beijing, China.
Front Microbiol. 2022 Jun 23;13:892437. doi: 10.3389/fmicb.2022.892437. eCollection 2022.
Fu Brick tea is a very popular post-fermented tea that is known for its "golden flower fungus," , which becomes the dominant microbe during the maturation process. This study used both culture-dependent methods and high-throughput sequencing to track microbial succession and interactions during the development of the golden flower fungus, a crucial component of the manufacturing process of Fu Brick tea. Among the bacterial communities, and were consistently cultured from both fresh tea leaves and in post-fermentation Fu Brick tea. , and were dominant genera in fresh tea leaves but declined once fermentation started, while , and became dominant after piling fermentation. The abundance of increased during the manufacturing process, accounting for over 98% of all fungi present after the golden flower bloom in the Fu Brick tea product. Despite their consistent presence during culture work, network analysis showed and to be negatively correlated with . spp., as expected from culture work, positively correlated with the presence of golden flower fungus. This study provides complete insights about the succession of microbial communities and highlights the importance of co-occurrence microbes with during the manufacturing process of Fu Brick tea.
茯砖茶是一种非常受欢迎的后发酵茶，以其“金花菌”而闻名，金花菌在成熟过程中成为主要微生物。本研究使用了基于培养的方法和高通量测序技术，来追踪金花菌（茯砖茶制造过程中的关键成分）发育过程中的微生物演替和相互作用。在细菌群落中，[具体细菌名称1]和[具体细菌名称2]在新鲜茶叶和后发酵茯砖茶中均能持续培养出来。[具体细菌名称3]、[具体细菌名称4]和[具体细菌名称5]是新鲜茶叶中的优势属，但发酵开始后数量下降，而[具体细菌名称6]、[具体细菌名称7]和[具体细菌名称8]在堆积发酵后成为优势菌属。[具体真菌名称]的丰度在制造过程中增加，在茯砖茶产品金花绽放后占所有真菌的98%以上。尽管在培养工作中它们一直存在，但网络分析表明[具体细菌名称9]和[具体细菌名称10]与[具体细菌名称11]呈负相关。正如培养工作所预期的，[具体细菌名称12] spp.与金花菌的存在呈正相关。本研究提供了关于微生物群落演替的完整见解，并突出了茯砖茶制造过程中与[具体细菌名称13]共生微生物的重要性。
