Turnbull P C, Richmond J E
Br J Exp Pathol. 1978 Feb;59(1):64-75.
Salmonella enteritidis was injected into isolated "loops" of duodenum, midgut and ileocaeca in anaesthetized 1-day-old chicks. Using light microscopy with conventional staining and fluorescent antibody techniques, the organism were observed in increasing numbers in the epithelium and lamina propria of each region between 2 and 12 h after inoculation. The potential ability of the salmonellas to enter the mucosa at any level of the intestine was thus demonstrated. The penetration of caecal epithelium by the organisms was studied by electron-microscopy. Uptake of salmonellas was associated with evaginations which formed at the luminal surface of the epithelium. In the epithelial cells, the bacteria were enclosed within membrane-bound vacuoles and appeared undamaged by intracellular passage. Surface evaginations almost completely replaced the brush border in regions where large numbers of the organisms were involved. Occasional salmonellas were found in the lamina propria both free and intracellularly. Results of culture and light microscopy following oral and intracloacal inoculation are also presented.
将肠炎沙门氏菌注射到麻醉的1日龄雏鸡分离的十二指肠、中肠和回盲部“肠袢”中。使用常规染色和荧光抗体技术的光学显微镜观察发现，接种后2至12小时内，每个区域的上皮和固有层中该菌数量不断增加。由此证明了沙门氏菌在肠道任何水平进入黏膜的潜在能力。通过电子显微镜研究了该菌对盲肠上皮的穿透情况。沙门氏菌的摄取与上皮腔面形成的外翻有关。在上皮细胞中，细菌被包裹在膜结合的液泡内，且在细胞内穿行时未受损。在大量细菌存在的区域，表面外翻几乎完全取代了刷状缘。在固有层中偶尔发现游离和细胞内的沙门氏菌。还呈现了经口和经泄殖腔接种后的培养结果和光学显微镜观察结果。
