用重金属对组织切片进行电子显微镜染色。
Staining of tissue sections for electron microscopy with heavy metals.
作者信息
WATSON M L
出版信息
J Biophys Biochem Cytol. 1958 Jul 25;4(4):475-8. doi: 10.1083/jcb.4.4.475.
Abstract
Heavy metals may be incorporated from solution into tissue sections for electron microscopy. The resulting increase in density of the tissue provides greatly enhanced contrast with minimal distortion. Relative densities of various structures are found to depend on the heavy metal ions present and on the conditions of staining. Certain hitherto unobserved details are revealed and some sort of specificity exists, although the factors involved are not yet understood.
摘要
重金属可从溶液中掺入组织切片用于电子显微镜检查。由此导致的组织密度增加能在最小程度的变形下极大地增强对比度。发现各种结构的相对密度取决于所存在的重金属离子以及染色条件。某些迄今未观察到的细节得以显现，并且存在某种特异性，尽管其中涉及的因素尚不清楚。
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