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环状PTK2通过靶向miR-196a-3p/AATK轴抑制胃癌进展。

CircPTK2 Suppresses the Progression of Gastric Cancer by Targeting the MiR-196a-3p/AATK Axis.

作者信息

Gao Ling, Xia Tingting, Qin Mingde, Xue Xiaofeng, Jiang Linhua, Zhu Xinguo

机构信息

Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, China.

Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Suzhou, China.

出版信息

Front Oncol. 2021 Sep 15;11:706415. doi: 10.3389/fonc.2021.706415. eCollection 2021.

DOI:10.3389/fonc.2021.706415
PMID:34604044
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8479173/
Abstract

BACKGROUND

Gastric cancer is a type of malignant tumor with high morbidity and mortality. It has been shown that circular RNAs (circRNAs) exert critical roles in gastric cancer progression working as microRNA (miRNA) sponges to regulate gene expression. However, the role and potential molecular mechanism of circRNAs in gastric cancer remain largely unknown.

METHODS

CircPTK2 (hsa_circ_0005273) was identified by bioinformatics analysis and validated by RT-qPCR assay. Bioinformatics prediction, dual-luciferase reporter, and RNA pull-down assays were used to determine the interaction between circPTK2, miR-196a-3p, and apoptosis-associated tyrosine kinase 1 (AATK).

RESULTS

The level of circPTK2 was markedly downregulated in gastric cancer tissues and gastric cancer cells. Upregulation of circPTK2 significantly suppressed the proliferation, migration, and invasion of gastric cancer cells, while circPTK2 knockdown exhibited opposite effects. Mechanically, circPTK2 could competitively bind to miR-196a-3p and prevent miR-196a-3p to reduce the expression of AATK. In addition, overexpression of circPTK2 inhibited tumorigenesis in a xenograft mouse model of gastric cancer.

CONCLUSION

Collectively, circPTK2 functions as a tumor suppressor to suppress gastric cancer cell proliferation, migration, and invasion through regulating the miR-196a-3p/AATK axis, suggesting that circPTK2 may serve as a novel therapeutic target for gastric cancer.

摘要

背景

胃癌是一种发病率和死亡率都很高的恶性肿瘤。研究表明，环状RNA（circRNA）作为微小RNA（miRNA）海绵发挥作用来调节基因表达，在胃癌进展中发挥关键作用。然而，circRNA在胃癌中的作用和潜在分子机制仍 largely未知。

方法

通过生物信息学分析鉴定circPTK2（hsa_circ_0005273），并通过RT-qPCR检测进行验证。使用生物信息学预测、双荧光素酶报告基因和RNA下拉实验来确定circPTK2、miR-196a-3p和凋亡相关酪氨酸激酶1（AATK）之间的相互作用。

结果

circPTK2水平在胃癌组织和胃癌细胞中明显下调。circPTK2的上调显著抑制了胃癌细胞的增殖、迁移和侵袭，而circPTK2敲低则表现出相反的效果。机制上，circPTK2可以竞争性结合miR-196a-3p并阻止miR-196a-3p降低AATK的表达。此外，circPTK2的过表达在胃癌异种移植小鼠模型中抑制了肿瘤发生。

结论

总的来说，circPTK2作为一种肿瘤抑制因子，通过调节miR-196a-3p/AATK轴来抑制胃癌细胞的增殖、迁移和侵袭，这表明circPTK2可能成为胃癌的一个新的治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e5/8479173/5ddde2fbcc9f/fonc-11-706415-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e5/8479173/4f3730d40962/fonc-11-706415-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e5/8479173/5cb88994d430/fonc-11-706415-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e5/8479173/9f4870e89b37/fonc-11-706415-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e5/8479173/15af0e2b8844/fonc-11-706415-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e5/8479173/0e905ac97ad8/fonc-11-706415-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e5/8479173/95a7aee853b1/fonc-11-706415-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e5/8479173/ec3030d5e9b4/fonc-11-706415-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e5/8479173/5ddde2fbcc9f/fonc-11-706415-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e5/8479173/4f3730d40962/fonc-11-706415-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e5/8479173/5cb88994d430/fonc-11-706415-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e5/8479173/9f4870e89b37/fonc-11-706415-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e5/8479173/15af0e2b8844/fonc-11-706415-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e5/8479173/0e905ac97ad8/fonc-11-706415-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e5/8479173/95a7aee853b1/fonc-11-706415-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e5/8479173/ec3030d5e9b4/fonc-11-706415-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94e5/8479173/5ddde2fbcc9f/fonc-11-706415-g008.jpg

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3
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