Dimén Diána, Puska Gina, Szendi Vivien, Sipos Eszter, Zelena Dóra, Dobolyi Árpád
MTA-ELTE Laboratory of Molecular and Systems Neurobiology, Department of Physiology and Neurobiology, Hungarian Academy of Sciences, Eötvös Loránd Research Network, Eötvös Loránd University, 1117 Budapest, Hungary.
Department of Ecology, University of Veterinary Medicine Budapest, 1078 Budapest, Hungary.
iScience. 2021 Sep 4;24(10):103090. doi: 10.1016/j.isci.2021.103090. eCollection 2021 Oct 22.
The role of preoptic GABAergic inhibitory neurons was addressed in parenting, anxiety and depression. Pup exposure and forced swimming resulted in similar activation pattern in neurons expressing vesicular GABA transporter in the preoptic area with generally stronger labeling and different distributional pattern in females than in males. Chemogenetic stimulation of preoptic GABAergic cells resulted in elevated maternal motivation and caring behavior in females and mothers but aggression toward pups in males. Behavioral effects were the opposite following inhibition of preoptic GABAergic neurons suggesting their physiological relevance. In addition, increased anxiety-like and depression-like behaviors were found following chemogenetic stimulation of the same neurons in females, whereas previous pup exposure increased only anxiety-like behavior suggesting that not the pups, but overstimulation of the cells can lead to depression-like behavior. A sexually dimorphic projection pattern of preoptic GABAergic neurons was also identified, which could mediate sex-dependent parenting and associated emotional behaviors.
视前区γ-氨基丁酸能抑制性神经元在养育行为、焦虑和抑郁方面的作用得到了研究。幼崽暴露和强迫游泳导致视前区表达囊泡γ-氨基丁酸转运体的神经元出现相似的激活模式,雌性的标记通常更强且分布模式与雄性不同。对视前区γ-氨基丁酸能细胞进行化学遗传学刺激,会使雌性和母亲的母性动机和关爱行为增强,但会导致雄性对幼崽产生攻击行为。对视前区γ-氨基丁酸能神经元进行抑制后,行为效应则相反,这表明了它们的生理相关性。此外,对雌性同一神经元进行化学遗传学刺激后,发现类似焦虑和抑郁的行为增加,而之前的幼崽暴露仅增加了类似焦虑的行为,这表明导致类似抑郁行为的不是幼崽,而是细胞的过度刺激。还确定了视前区γ-氨基丁酸能神经元的性别二态投射模式,这可能介导了性别依赖性的养育行为及相关情绪行为。