孕酮纳米粒对弱精子症患者精子获能和顶体反应的影响。

Effects of progesterone nanoparticles on the sperm capacitation and acrosome reaction in asthenozoospermia men.

机构信息

Medicine school, Department of Clinical Biochemistry, Hamadan University of Medical Sciences, Hamadan, Iran.

School of Pharmacy, Department of Pharmaceutics and Pharmaceutical Nanotechnology, Hamadan University of Medical Sciences, Hamadan, Iran.

出版信息

Andrologia. 2022 Feb;54(1):e14258. doi: 10.1111/and.14258. Epub 2021 Oct 5.

DOI:10.1111/and.14258

PMID:34609765

Abstract

Low motility is one of the causes of male infertility. In this study, the effects of progesterone solid lipid nanoparticles (SLNs) on sperm capacitation, acrosome reaction, oxidative stress and expression of SPACA1 and MAPK way genes were investigated. Progesterone SLNs were synthesized using the solvent emulsification evaporation method. Twenty asthenozoospermia samples were selected, and sperm and acrosome membrane integrity, acrosome reaction, sperm motility, viability, total antioxidant capacity (TAC), total oxidative status tests and PKA, PTK, P38MAPK and SPACA1 gene expressions were assessed. The synthesized nanoparticles were prepared with the size (187.6 nm), PDI (0.184), EE (85.82%), LP (3.43%) and ZP (-23.5mV). Progesterone SLNs increased sperm and acrosome membrane integrity and TAC (p < .05). Also, the expression of P38MAPK, PKA, PTK, and SPACA1 genes in this group showed a significant increase (p < .001). Progesterone SLNs increased acrosome reaction, sperm capacitation and TAC. Also, it increased the expression of PTK PKA, SPACA1 and P38MAPK genes.

摘要

精子活力低下是男性不育的原因之一。本研究探讨了孕酮固体脂质纳米粒（SLN）对精子获能、顶体反应、氧化应激以及 SPACA1 和 MAPK 途径基因表达的影响。采用溶剂乳化蒸发法合成孕酮 SLN。选取 20 例弱精子症样本，评估精子和顶体膜完整性、顶体反应、精子活力、存活率、总抗氧化能力（TAC）、总氧化状态试验以及 PKA、PTK、P38MAPK 和 SPACA1 基因表达。合成的纳米粒大小为（187.6nm）、PDI（0.184）、EE（85.82%）、LP（3.43%）和 ZP（-23.5mV）。孕酮 SLN 增加了精子和顶体膜的完整性和 TAC（p<0.05）。此外，该组中 P38MAPK、PKA、PTK 和 SPACA1 基因的表达也显著增加（p<0.001）。孕酮 SLN 增加了顶体反应、精子获能和 TAC。同时，它还增加了 PTK、PKA、SPACA1 和 P38MAPK 基因的表达。