Faculty of Medicine, Department of Histology and Embryology, Gaziantep University, Gaziantep, Turkey.
Faculty of Medicine, Department of Histology-Embryology, Manisa Celal Bayar University, Manisa, Turkey; and DESAM Research Institute, Near East University, Mersin 10, Turkey.
Reprod Fertil Dev. 2021 Nov;33(16):799-809. doi: 10.1071/RD21140.
The purpose of this study was to investigate the effect of kisspeptin-54 on ovarian morphology and vascular endothelial growth factor (VEGF), pigment epithelium-derived factor (PEDF), protein kinase A (PKA) and protein kinase C (PKC) levels in an ovarian hyperstimulation syndrome (OHSS) rat model, which is a possible complication of controlled ovarian hyperstimulation. For this purpose, immature female Sprague-Dawley rats (25days old, 30-40g) were randomly divided into five groups (control, sham, OHSS model, short-term kisspeptin-54 administered OHSS model and long-term kisspeptin-54 administered OHSS model). Serum LH and FSH levels were determined by enzyme-linked immunosorbent assay. Immunohistochemistry and quantitative RT-PCR were performed for VEGF, PEDF, PKA and PKC in ovaries and granulosa cells, respectively. It was observed that there was dilatation in fallopian tubes and an abnormal increase in ovarian weight and volume in the OHSS group, and these morphologies decreased with kisspeptin-54 treatment. After the administration of kisspeptin-54 in the OHSS group, VEGF, PKA and PKC levels reduced and PEDF levels increased in both mRNA and protein levels. It was determined that in the OHSS model, VEGF increased as PEDF decreased, and kisspeptin-54 reduced the effects of OHSS. It was determined that long-term kisspeptin-54 treatment was more effective than short-term administration. It is considered that kisspeptin-54 is an agent that protects ovarian reserve and oocyte maturation in women at risk of OHSS.
本研究旨在探讨 kisspeptin-54 对卵巢过度刺激综合征（OHSS）大鼠模型中卵巢形态和血管内皮生长因子（VEGF）、色素上皮衍生因子（PEDF）、蛋白激酶 A（PKA）和蛋白激酶 C（PKC）水平的影响，OHSS 是一种控制性卵巢过度刺激的可能并发症。为此，将未成熟雌性 Sprague-Dawley 大鼠（25 天大，30-40g）随机分为五组（对照组、假手术组、OHSS 模型组、短期 kisspeptin-54 给药 OHSS 模型组和长期 kisspeptin-54 给药 OHSS 模型组）。通过酶联免疫吸附试验测定血清 LH 和 FSH 水平。分别对卵巢和颗粒细胞中的 VEGF、PEDF、PKA 和 PKC 进行免疫组织化学和定量 RT-PCR。结果观察到，OHSS 组输卵管扩张，卵巢重量和体积异常增加，给予 kisspeptin-54 治疗后这些形态学变化减少。在 OHSS 组给予 kisspeptin-54 后，VEGF、PKA 和 PKC 水平降低，PEDF 水平在 mRNA 和蛋白水平均增加。结果表明，在 OHSS 模型中，VEGF 随着 PEDF 的减少而增加，而 kisspeptin-54 降低了 OHSS 的作用。确定长期 kisspeptin-54 治疗比短期给药更有效。认为 kisspeptin-54 是一种保护卵巢储备和卵子成熟的药物，可用于有发生 OHSS 风险的女性。
