Pereira M A, Klaunig J E, Herren-Freund S L, Ruch R J
J Natl Cancer Inst. 1986 Aug;77(2):449-52.
The effect of long-term exposure to phenobarbital (CAS: 50-06-6) subsequent to tumor initiation on the development of liver tumors in BALB/c and (C57BL/6 X C3H/Anf)F1 (B6C3F1) mice was determined. In male B6C3F1 mice that received either 15 or 45 ppm diethylnitrosamine [(DENA) CAS: 55-18-5] between 6 and 10 weeks of age, subsequent treatment with 500 ppm sodium phenobarbital in the drinking water resulted in the promotion of liver tumors. However, in male B6C3F1 mice initiated on day 15 of age with 25 mg DENA/kg, beginning long-term treatment of 500 ppm sodium phenobarbital at 4 weeks of age inhibited the development of liver tumors, whereas in male BALB/c mice initiated with 25 mg DENA/kg on day 15 of age, beginning the long-term treatment with 500 ppm sodium phenobarbital at 4 weeks of age promoted the development of liver tumors. Hence phenobarbital can either enhance or inhibit the formation of liver tumors, depending both on the mouse strain used and the animal's age at the start of exposure.
确定了在肿瘤引发后长期暴露于苯巴比妥（CAS：50 - 06 - 6）对BALB/c和（C57BL/6×C3H/Anf）F1（B6C3F1）小鼠肝脏肿瘤发生发展的影响。在6至10周龄期间接受15或45 ppm二乙基亚硝胺[（DENA）CAS：55 - 18 - 5]的雄性B6C3F1小鼠中，随后在饮用水中给予500 ppm苯巴比妥钠导致肝脏肿瘤的促进。然而，在15日龄时用25 mg DENA/kg引发的雄性B6C3F1小鼠中，4周龄开始长期给予500 ppm苯巴比妥钠抑制了肝脏肿瘤的发展，而在15日龄时用25 mg DENA/kg引发的雄性BALB/c小鼠中，4周龄开始长期给予500 ppm苯巴比妥钠促进了肝脏肿瘤的发展。因此，苯巴比妥既可以增强也可以抑制肝脏肿瘤的形成，这取决于所使用的小鼠品系以及暴露开始时动物的年龄。
