Department of Stomatology, Wuhan Dongxihu District People's Hospital, Wuhan, Hubei, China.
Bioengineered. 2021 Dec;12(1):7907-7919. doi: 10.1080/21655979.2021.1977103.
Long non-coding RNA (lncRNA) CASC9 is reported to be a tumor promoter in oral cancer, but its mechanism in oral squamous cell carcinoma (OSCC) has not been fully explored. Our study aimed to identify the interaction between lncRNA CASC9, microRNA-545-3p (miR-545-3p), and laminin subunit gamma 2 (LAMC2) in OSCC cells. Our study confirmed that lncRNA CASC9 and LAMC2 were upregulated in OSCC, whereas miR-545-3p expression was reduced. After performing a series of cell functional experiments, it was found that knockdown of lncRNA CASC9 or LAMC2 resulted in the inhibition of proliferation, colony formation, and migration of OSCC cells, but their negative effects could be partly impaired by the miR-545-3p inhibitor. In addition, we proved for the first time that lncRNA CASC9 can sponge miR-545-3p to upregulate LAMC2. In conclusion, our study revealed that lncRNA CASC9 promotes the malignancy of OSCC cells by sponging miR-545-3p to enhance LAMC2 expression, implying that lncRNA CASC9/miR-545-3p/LAMC2 may be an intervention approach in OSCC therapy.
长链非编码 RNA（lncRNA）CASC9 被报道在口腔癌中是一种肿瘤促进物，但它在口腔鳞状细胞癌（OSCC）中的机制尚未被充分探索。我们的研究旨在确定 lncRNA CASC9、微小 RNA-545-3p（miR-545-3p）和层粘连蛋白亚单位 γ2（LAMC2）在 OSCC 细胞中的相互作用。我们的研究证实，lncRNA CASC9 和 LAMC2 在 OSCC 中上调，而 miR-545-3p 的表达降低。进行了一系列细胞功能实验后，发现敲低 lncRNA CASC9 或 LAMC2 导致 OSCC 细胞的增殖、集落形成和迁移受到抑制，但 miR-545-3p 抑制剂可部分削弱其负作用。此外，我们首次证明 lncRNA CASC9 可以通过海绵吸附 miR-545-3p 来上调 LAMC2。总之，我们的研究揭示了 lncRNA CASC9 通过海绵吸附 miR-545-3p 来促进 OSCC 细胞的恶性转化，表明 lncRNA CASC9/miR-545-3p/LAMC2 可能是 OSCC 治疗的一种干预方法。
