Zhang Yongjing, Zhang Aijing, Li Mengyuan, He Kanglai, Guo Shuyuan
School of Life Science, Beijing Institute of Technology, Beijing, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
J Microencapsul. 2021 Nov-Dec;38(7-8):522-532. doi: 10.1080/02652048.2021.1990424. Epub 2021 Oct 19.
To prepare several novel microcapsules using chitosan (Cs) and Alginate (Alg) as coating materials, and nano-ZnO, nano-SiO, nano-TiO as UV protective agents for improving UV resistance of Cry1Ac.
Microcapsules were prepared by the layer-by-layer (LbL) self-assembly technique and electrostatic adsorption. The morphologies were observed by scanning electron microscopy (SEM), and the stability under UV radiation was studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and bioassay.
SEM showed that nano-ZnO and nano-TiO could be adsorbed on the negatively charged MC with the outermost layer being Alg, while nano-SiO could be adsorbed on the positively charged MC with Cs as the outermost layer. SDS-PAGE and bioassay showed that nano-ZnO and nano-SiO could provide effective UV protection after 8 h UV irradiation ( > 0.05), and nano-TiO could provide effective UV protection after 4 h UV irradiation ( > 0.05).
The microcapsules loaded with nanoparticles provided excellent UV resistance for Cry1Ac.
以壳聚糖（Cs）和海藻酸钠（Alg）为包衣材料，纳米氧化锌（nano-ZnO）、纳米二氧化硅（nano-SiO₂）、纳米二氧化钛（nano-TiO₂）为紫外线防护剂，制备几种新型微胶囊，以提高Cry1Ac的抗紫外线能力。
采用层层（LbL）自组装技术和静电吸附法制备微胶囊。通过扫描电子显微镜（SEM）观察其形态，并通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和生物测定研究其在紫外线辐射下的稳定性。
SEM显示，纳米氧化锌和纳米二氧化钛可吸附在带负电荷的微胶囊上，最外层为海藻酸钠，而纳米二氧化硅可吸附在带正电荷的微胶囊上，最外层为壳聚糖。SDS-PAGE和生物测定表明，纳米氧化锌和纳米二氧化硅在紫外线照射8小时后能提供有效的紫外线防护（P>0.05），纳米二氧化钛在紫外线照射4小时后能提供有效的紫外线防护（P>0.05）。
负载纳米颗粒的微胶囊为Cry1Ac提供了优异的抗紫外线能力。
