Austgulen R, Hammerstrøm J, Espevik T, Nissen-Meyer J
Cell Immunol. 1986 Mar;98(1):211-20. doi: 10.1016/0008-8749(86)90281-9.
Human monocytes can be activated to release a 40,000-Da cytostatic protein factor (CF). In this report we have investigated the cytolytic activity of CF on WEHI 164 cells which are sensitive to monocyte-mediated cytolysis. Monocyte supernatants containing CF induced cytolysis of murine WEHI 164 sarcoma cells, as determined in a 51Cr-release assay. Preincubation of WEHI 164 cells with actinomycin D enhanced cytolysis induced by supernatants containing CF, suggesting that CF may be involved in drug-dependent cellular cytotoxicity. The cytolytic activity was profoundly inhibited by a rabbit antiserum raised against purified CF, indicating that the cytolytic activity in the supernatants was in fact mediated by CF. These results indicate that CF may be an important effector molecule in monocyte-mediated cytostatic and cytolytic reactions.
人单核细胞可被激活以释放一种40000道尔顿的细胞生长抑制蛋白因子（CF）。在本报告中，我们研究了CF对WEHI 164细胞的细胞溶解活性，该细胞对单核细胞介导的细胞溶解敏感。在51Cr释放试验中测定，含有CF的单核细胞上清液可诱导鼠WEHI 164肉瘤细胞的细胞溶解。用放线菌素D预孵育WEHI 164细胞可增强含有CF的上清液诱导的细胞溶解，这表明CF可能参与了药物依赖性细胞毒性作用。针对纯化的CF产生的兔抗血清可显著抑制细胞溶解活性，这表明上清液中的细胞溶解活性实际上是由CF介导的。这些结果表明，CF可能是单核细胞介导的细胞生长抑制和细胞溶解反应中的一种重要效应分子。
