Beran M, Hittelman W N, Andersson B S, McCredie K B
Leuk Res. 1986;10(8):1033-9. doi: 10.1016/0145-2126(86)90256-0.
Normalization of granulocyte counts was obtained with low-dose cytosine arabinoside (Ara-C) in a patient with acute myelogenous leukemia (AML, FAB-M2) who had relapsed on maintenance therapy containing conventional doses of Ara-C and was clinically resistant to high-dose Ara-C treatment. The patient's leukemic cells exhibited an abnormal karyotype (45, X-Y, t[8q; 21q]). To determine whether the response to low-dose Ara-C was a result of induced leukemic cell differentiation, the mature peripheral blood granulocytes were isolated and fused with mitotic chinese hamster ovary (CHO) cells to induce premature chromosome condensation. Karyotypic evaluation of the granulocyte prematurely condensed chromosomes (PCC) during response to low-dose Ara-C demonstrated the presence of cells with 45 chromosomes. This result strongly suggests that part of this patient's response to low-dose Ara-C was a result of induced maturation of the leukemic clone.
一名急性髓系白血病（AML，FAB-M2）患者在接受含常规剂量阿糖胞苷（Ara-C）的维持治疗时复发，且对高剂量Ara-C治疗产生临床耐药，使用低剂量阿糖胞苷（Ara-C）后粒细胞计数恢复正常。该患者的白血病细胞表现出异常核型（45, X-Y, t[8q; 21q]）。为了确定对低剂量Ara-C的反应是否是诱导白血病细胞分化的结果，分离出成熟的外周血粒细胞并与有丝分裂的中国仓鼠卵巢（CHO）细胞融合，以诱导染色体早熟凝集。对低剂量Ara-C反应期间粒细胞早熟凝集染色体（PCC）的核型评估显示存在45条染色体的细胞。这一结果有力地表明，该患者对低剂量Ara-C的部分反应是白血病克隆诱导成熟的结果。
