Changes in weight gain, digestive and metabolic enzyme activities in Labeo rohita fingerlings in response to multiple stress exposure and dietary nutraceutical.

A feeding trial was conducted for 4 months to study the effect of a nutraceutical conglomerate at different levels (0, 0.1, and 0.5%) on stress-exposed (high stocking density, H and feed restriction, R) Labeo rohita fingerlings. Six isonitrogenous diets with a crude protein of 35% were prepared for the different treatments, viz. THR0, THR1, THR5, TNS0, TNS1 and TNS5. Stress significantly reduced the weight gain and feeding of 0.1% nutraceutical improved it in both stress-exposed and non-exposed groups from 3 months onwards. Two-and three-month stress-exposed groups exhibited lower (p < 0.05) protease activity, while amylase activity was significantly higher in 2-month stress-exposed groups. Higher lipase activity was found in stress-exposed groups irrespective of the duration of stress. Higher serum glutathione peroxidase (GPx), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and hepatic and muscle lactate dehydrogenase (LDH) activities were observed in the THR0 groups; however, THR1 showed a lower activity of these enzymes. Increased malate dehydrogenase (MDH) and glucose-6-phosphate dehydrogenase (G6PDH) activity was recorded in the 1st and 2nd months stress-exposed groups. However, THR1 group exhibited a reduced MDH and G6PDH activity compared to THR0 group in 1st and 2nd months. Hence, it can be concluded that the duration of multiple stress exposure adversely affected the digestive and metabolic enzymes activities and feeding of 0.1% nutraceutical conglomerates could restore the activities of digestive and metabolic enzymes in rohu fingerlings.