Kumar Deepak, Bhattacharyya Rajasri, Banerjee Dibyajyoti
Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India.
Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India.
Anal Biochem. 2021 Nov 15;633:114402. doi: 10.1016/j.ab.2021.114402. Epub 2021 Oct 7.
Detection of microalbuminuria is an analytical challenge. There are dye-based methods and immunochemical methods. However, these methods are less specific and sensitive respectively. So, people are trying new approaches for microalbuminuria detection. In this context, we have developed a fluorescent spectroscopic method to detect human serum albumin using its pseudoesterase property. Recently, we had discovered that neostigmine does not inhibit Human serum albumin pseudoesterase activity. Using such a phenomenon, we have devised a specific fluorimetric detection method of HSA using 2NA as a substrate for the pseudoesterase activity. The developed method can sense as low as 0.1 μM of HSA in the urine matrix without dye or antibody. We have proposed a scheme of automation of the proposed method.
微量白蛋白尿的检测是一项分析挑战。有基于染料的方法和免疫化学方法。然而，这些方法分别具有较低的特异性和敏感性。因此，人们正在尝试用于微量白蛋白尿检测的新方法。在此背景下，我们开发了一种荧光光谱法，利用人血清白蛋白的拟酯酶特性来检测人血清白蛋白。最近，我们发现新斯的明不会抑制人血清白蛋白拟酯酶活性。利用这一现象，我们设计了一种以2NA作为拟酯酶活性底物的特异性荧光检测人血清白蛋白的方法。所开发的方法在无染料或抗体的尿液基质中能够检测低至0.1 μM的人血清白蛋白。我们提出了该方法的自动化方案。
