Faculty of Engineering and Natural Sciences, Tampere University, P.O. Box 541, FI-33104 Tampere University, Finland; UNESCO-IHE, Institute for Water Education, Westvest 7, 2611AX Delft, the Netherlands; School of Chemical and Biological Sciences, and Ryan Institute, National University of Ireland Galway, University Road, Galway H91 TK33, Ireland.
Faculty of Engineering and Natural Sciences, Tampere University, P.O. Box 541, FI-33104 Tampere University, Finland.
Bioresour Technol. 2022 Jan;343:126098. doi: 10.1016/j.biortech.2021.126098. Epub 2021 Oct 7.
Distinct microbial assemblages evolve in anaerobic digestion (AD) reactors to drive sequential conversions of organics to methane. The spatio-temporal development of three such assemblages (granules, biofilms, planktonic) derived from the same inoculum was studied in replicated bioreactors treating long-chain fatty acids (LCFA)-rich wastewater at 20 °C at hydraulic retention times (HRTs) of 12-72 h. We found granular, biofilm and planktonic assemblages differentiated by diversity, structure, and assembly mechanisms; demonstrating a spatial compartmentalisation of the microbiomes from the initial community reservoir. Our analysis linked abundant Methanosaeta and Syntrophaceae-affiliated taxa (Syntrophus and uncultured) to their putative, active roles in syntrophic LCFA bioconversion. LCFA loading rates (stearate, palmitate), and HRT, were significant drivers shaping microbial community dynamics and assembly. This study of the archaea and syntrophic bacteria actively valorising LCFAs at short HRTs and 20 °C will help uncover the microbiology underpinning anaerobic bioconversions of fats, oil and grease.
在厌氧消化（AD）反应器中，不同的微生物群落演化以驱动有机物到甲烷的连续转化。本研究从相同接种物中衍生的三种群落（颗粒、生物膜、浮游）在复制的生物反应器中进行研究，这些生物反应器在 20°C 下用水力停留时间（HRT）为 12-72 h 处理富含长链脂肪酸（LCFA）的废水。我们发现颗粒、生物膜和浮游群落的多样性、结构和组装机制不同；这表明微生物组从初始群落库中具有空间分隔。我们的分析将丰富的产甲烷菌和与营养相关的科（Syntrophaceae 相关）的分类群（Syntrophus 和未培养）与其在协同 LCFA 生物转化中的潜在活性作用联系起来。LCFA 加载速率（硬脂酸盐、棕榈酸盐）和 HRT 是影响微生物群落动态和组装的重要驱动因素。本研究对在短 HRT 和 20°C 下积极利用 LCFAs 的古菌和协同细菌进行了研究，这将有助于揭示脂肪、油和油脂的厌氧生物转化的微生物学基础。
