串联质谱法进行蛋白质测序
Protein sequencing by tandem mass spectrometry.
作者信息
Hunt D F, Yates J R, Shabanowitz J, Winston S, Hauer C R
出版信息
Proc Natl Acad Sci U S A. 1986 Sep;83(17):6233-7. doi: 10.1073/pnas.83.17.6233.
Abstract
Methodology for determining amino acid sequences of proteins by tandem mass spectrometry is described. The approach involves enzymatic and/or chemical degradation of the protein to a collection of peptides which are then fractionated by high-performance liquid chromatography. Each fraction, containing as many as 10-15 peptides, is then analyzed directly, without further purification, by a combination of liquid secondary-ion/collision-activated dissociation mass spectrometry on a multianalyzer instrument. Interpretation of collision-activated dissociation mass spectra is described, and results are presented from a study of soluble peptides produced by treatment of apolipoprotein B with cyanogen bromide and trypsin.
摘要
描述了通过串联质谱法测定蛋白质氨基酸序列的方法。该方法包括将蛋白质进行酶促和/或化学降解，得到一系列肽段，然后通过高效液相色谱法对这些肽段进行分离。每个含有多达10 - 15个肽段的馏分，随后在多分析仪仪器上通过液相二次离子/碰撞激活解离质谱联用直接进行分析，无需进一步纯化。文中描述了对碰撞激活解离质谱的解读，并给出了对用溴化氰和胰蛋白酶处理载脂蛋白B产生的可溶性肽段进行研究的结果。
相似文献
1
Protein sequencing by tandem mass spectrometry.串联质谱法进行蛋白质测序
Proc Natl Acad Sci U S A. 1986 Sep;83(17):6233-7. doi: 10.1073/pnas.83.17.6233.
2
Mass spectrometric analysis of integral membrane proteins: application to complete mapping of bacteriorhodopsins and rhodopsin.整合膜蛋白的质谱分析：应用于细菌视紫红质和视紫红质的完整图谱绘制
Protein Sci. 1998 Mar;7(3):758-64. doi: 10.1002/pro.5560070325.
3
Rapid identification of comigrating gel-isolated proteins by ion trap-mass spectrometry.通过离子阱质谱快速鉴定凝胶分离的共迁移蛋白质。
Electrophoresis. 1998 May;19(6):968-80. doi: 10.1002/elps.1150190612.
4
Formation of c1 fragment ions in collision-induced dissociation of glutamine-containing peptide ions: a tip for de novo sequencing.含谷氨酰胺肽离子碰撞诱导解离中C1碎片离子的形成：从头测序的一个提示
Rapid Commun Mass Spectrom. 2004;18(18):2069-76. doi: 10.1002/rcm.1593.
5
Selective detection of lactolated peptides in hydrolysates by liquid chromatography/electrospray tandem mass spectrometry.通过液相色谱/电喷雾串联质谱法对水解产物中乳糖化肽进行选择性检测。
Anal Biochem. 1998 May 15;259(1):152-61. doi: 10.1006/abio.1998.2636.
6
Mass spectrometric de novo sequencing of natural non-tryptic peptides: comparing peculiarities of collision-induced dissociation (CID) and high energy collision dissociation (HCD).天然非胰蛋白酶解肽段的质谱从头测序：比较碰撞诱导解离（CID）和高能碰撞解离（HCD）的特点
Rapid Commun Mass Spectrom. 2014 Dec 15;28(23):2595-604. doi: 10.1002/rcm.7049.
7
[Mass-spectrometry with ion extraction from solution at atmospheric pressure for peptide mapping].
Bioorg Khim. 1988 Mar;14(3):293-8.
8
Ultrahigh-resolution Fourier transform ion cyclotron resonance mass spectrometry and tandem mass spectrometry for peptide de novo amino acid sequencing for a seven-protein mixture by paired single-residue transposed Lys-N and Lys-C digestion.通过配对单残基转位Lys-N和Lys-C消化，利用超高分辨率傅里叶变换离子回旋共振质谱和串联质谱对七种蛋白质混合物进行肽段从头氨基酸测序。
Rapid Commun Mass Spectrom. 2017 Jan 30;31(2):207-217. doi: 10.1002/rcm.7783.
9
The primary structure of thioredoxin from Chromatium vinosum determined by high-performance tandem mass spectrometry.
Biochemistry. 1987 Mar 10;26(5):1209-14. doi: 10.1021/bi00379a001.
10
Determination of the molecular mass of apolipoprotein B-100. A chemical approach.载脂蛋白B-100分子量的测定：一种化学方法
Biochem J. 1986 Nov 1;239(3):777-80. doi: 10.1042/bj2390777.
引用本文的文献
1
Professor D.F. Hunt Relationship with Finnigan Corporation and His Impact on Mass Spectrometer Development.D.F. 亨特教授与菲尼根公司的关系及其对质谱仪发展的影响。
Mol Cell Proteomics. 2025 Jul 16;24(9):101033. doi: 10.1016/j.mcpro.2025.101033.
2
Quantitative neuropeptide analysis by mass spectrometry: advancing methodologies for biological discovery.通过质谱法进行神经肽定量分析：推动生物学发现的方法进展
RSC Chem Biol. 2025 Jun 12. doi: 10.1039/d5cb00082c.
3
Serine Octamer Clusters Direct the Chirality of Peptides Formed in Water Microdroplets.丝氨酸八聚体簇决定了在水微滴中形成的肽的手性。
J Phys Chem A. 2025 May 22;129(20):4535-4542. doi: 10.1021/acs.jpca.5c00473. Epub 2025 May 8.
4
The Hunt Lab Weighs in on Mass Spectrometry-Based Analysis of Protein Posttranslational Modifications.亨特实验室对基于质谱的蛋白质翻译后修饰分析发表意见。
Mol Cell Proteomics. 2025 Apr;24(4):100943. doi: 10.1016/j.mcpro.2025.100943. Epub 2025 Mar 11.
5
Ion Mobility-Assisted Free Radical-Initiated Peptide Sequencing.离子淌度辅助自由基引发的肽测序
Int J Mass Spectrom. 2025 Feb;508. doi: 10.1016/j.ijms.2024.117396. Epub 2024 Dec 31.
6
Selected Ion Extraction of Peptides with Heavy Isotopes and Hydrogen Loss Reduces the Type II Error in Plasma Proteomics.具有重同位素和氢损失的肽的选择离子提取减少了血浆蛋白质组学中的II型错误。
ACS Omega. 2025 Jan 2;10(1):281-293. doi: 10.1021/acsomega.4c05624. eCollection 2025 Jan 14.
7
Risk assessment of homologous variants of biotech trait proteins using a bridging approach.利用桥接方法评估生物技术特性蛋白的同源变异体的风险。
GM Crops Food. 2024 Dec 31;15(1):336-351. doi: 10.1080/21645698.2024.2420412. Epub 2024 Nov 9.
8
The Hunt Lab Guide to De Novo Peptide Sequence Analysis by Tandem Mass Spectrometry.《亨特实验室串联质谱法从头肽序列分析指南》
Mol Cell Proteomics. 2024 Dec;23(12):100875. doi: 10.1016/j.mcpro.2024.100875. Epub 2024 Nov 7.
9
A Donald F. Hunt Story (John's Version).一个唐纳德·F·亨特的故事（约翰版）。
Mol Cell Proteomics. 2024 Dec;23(12):100869. doi: 10.1016/j.mcpro.2024.100869. Epub 2024 Oct 24.
10
Mass Spectrometry-Based Proteomics for the Masses: Peptide and Protein Identification in the Hunt Laboratory During the 2000's.面向大众的基于质谱的蛋白质组学：21世纪初亨特实验室中的肽和蛋白质鉴定
Mol Cell Proteomics. 2024 Dec;23(12):100866. doi: 10.1016/j.mcpro.2024.100866. Epub 2024 Oct 22.
本文引用的文献
1
Mixture analysis by triple-quadrupole mass spectrometry: metabolic profiling of urinary carboxylic acids.
Clin Chem. 1982 Dec;28(12):2387-92.
2
FAB-mapping of recombinant-DNA protein products.重组DNA蛋白产物的FAB图谱分析。
Biochem Biophys Res Commun. 1983 Nov 30;117(1):299-305. doi: 10.1016/0006-291x(83)91575-9.
3
Proposal for a common nomenclature for sequence ions in mass spectra of peptides.肽质谱序列离子通用命名法的建议。
Biomed Mass Spectrom. 1984 Nov;11(11):601. doi: 10.1002/bms.1200111109.
4
Human apolipoprotein B: partial amino acid sequence.人类载脂蛋白B：部分氨基酸序列。
FEBS Lett. 1984 May 7;170(1):105-8. doi: 10.1016/0014-5793(84)81378-2.
5
Strategy for the mass spectrometric verification and correction of the primary structures of proteins deduced from their DNA sequences.从DNA序列推导蛋白质一级结构的质谱验证和校正策略。
Proc Natl Acad Sci U S A. 1984 Apr;81(7):1956-60. doi: 10.1073/pnas.81.7.1956.
6
Peptide sequencing using the combination of edman degradation, carboxypeptidase digestion and fast atom bombardment mass spectrometry.使用埃德曼降解法、羧肽酶消化法和快原子轰击质谱法相结合的肽测序。
Biochem Biophys Res Commun. 1982 Feb 26;104(4):1223-30. doi: 10.1016/0006-291x(82)91381-x.
7
A partial cDNA clone for human apolipoprotein B.人载脂蛋白B的部分互补DNA克隆。
Proc Natl Acad Sci U S A. 1985 Aug;82(15):4983-6. doi: 10.1073/pnas.82.15.4983.
8
Cloning and expression of apolipoprotein B, the major protein of low and very low density lipoproteins.载脂蛋白B的克隆与表达，低密度和极低密度脂蛋白的主要蛋白质
Proc Natl Acad Sci U S A. 1985 Jul;82(14):4597-601. doi: 10.1073/pnas.82.14.4597.
9
Isolation of a cDNA clone encoding the amino-terminal region of human apolipoprotein B.编码人载脂蛋白B氨基末端区域的cDNA克隆的分离
Proc Natl Acad Sci U S A. 1986 Mar;83(5):1467-71. doi: 10.1073/pnas.83.5.1467.
10
Human apolipoprotein B-100: cloning, analysis of liver mRNA, and assignment of the gene to chromosome 2.人载脂蛋白B - 100：克隆、肝脏信使核糖核酸分析以及该基因在2号染色体上的定位
Proc Natl Acad Sci U S A. 1985 Dec;82(24):8340-4. doi: 10.1073/pnas.82.24.8340.
Zotero 插件