[长链非编码RNA ITGB2-AS1的过表达预示急性髓系白血病的不良预后]

[Overexpression of LncRNA ITGB2-AS1 Predicts Adverse Prognosis in Acute Myeloid Leukemia].

作者信息

Zhou Ying-Li, Xu Zi-Jun, Zhou Jing-Dong, Zhang Ting-Juan, Yao Dong-Ming, Ma Ji-Chun, Lin Jiang, Qian Jun

机构信息

School of Medicine, Jiangsu University, Zhenjiang 212013, Jiangsu Province, China,Department of Hematology, Affiliated People's Hospital of Jiangsu University, Zhenjiang 212002, Jiangsu Province, China,Zhenjiang Clinical Medical Research Center of Hematology, Zhenjiang 212002, Jiangsu Province, China,The Key Laboratory of Precision Diagnosis and Treatment of Zhenjiang City, Zhenjiang 212002, Jiangsu Province, China,Key Laboratory of Experimental Hematology, Affiliated People's Hospital of Jiangsu University, Zhenjiang 212002, Jiangsu Province, China.

出版信息

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2021 Oct;29(5):1436-1449. doi: 10.19746/j.cnki.issn.1009-2137.2021.05.011.

DOI:10.19746/j.cnki.issn.1009-2137.2021.05.011

PMID:34627422

Abstract

OBJECTIVE

LncRNA ITGB2-AS1 has been found to play important roles in the occurrence and development of human solid tumors. However, its role in hematological diseases, especially acute myeloid leukemia (AML), remains unclear. The aim of this study was to identify the expression pattern of ITGB2-AS1 in AML patients and to further explore its clinical significance.

METHODS

ITGB2-AS1 expression was analyzed in public datasets (including TCGA and GSE63270) and further validated in a cohort of 109 AML patients by real-time quantitative PCR (RT-qPCR).

RESULTS

The level of ITGB2-AS1 was up-regulated among two independent cohorts (TCGA, P<0.05; GSE63270, P<0.05), which was confirmed by the data from 109 AML patients enrolled in this study (P<0.05). Clinically, high ITGB2-AS1 expression was associated with older age (P=0.023) and lower complete remission (CR) rate (P=0.005). Multivariate analysis identified that high ITGB2-AS1 expression was an independent prognostic factor not only for CR rate (P=0.027) but also for overall survival (OS) time (P=0.011), and ITGB2-AS1 was positively correlated with ITGB2 expression in both TCGA (r=0.74, P<0.001) and clinical data detected in this study (r=0.881, P<0.001). High ITGB2 expression was also associated with older age (P=0.02) and lower CR rate (P=0.020). Moreover, high ITGB2 expression predicted worse OS (P=0.028).

CONCLUSION

ITGB2-AS1 is overexpressed in AML and predicts poor prognosis in AML patients.

摘要

目的

长链非编码RNA ITGB2-AS1已被发现参与人类实体瘤的发生发展过程。然而，其在血液系统疾病尤其是急性髓系白血病（AML）中的作用尚不清楚。本研究旨在明确ITGB2-AS1在AML患者中的表达模式，并进一步探讨其临床意义。

方法

在公共数据集（包括TCGA和GSE63270）中分析ITGB2-AS1的表达情况，并通过实时定量PCR（RT-qPCR）在109例AML患者队列中进行进一步验证。

结果

在两个独立队列（TCGA，P<0.05；GSE63270，P<0.05）中ITGB2-AS1水平上调，本研究纳入的109例AML患者的数据也证实了这一点（P<0.05）。临床上，ITGB2-AS1高表达与年龄较大（P=0.023）和完全缓解（CR）率较低（P=0.005）相关。多因素分析表明，ITGB2-AS1高表达不仅是CR率（P=0.027）的独立预后因素，也是总生存（OS）时间（P=0.011）的独立预后因素，且在TCGA（r=0.74，P<0.001）和本研究检测的临床数据中（r=0.881，P<0.001），ITGB2-AS1与ITGB2表达呈正相关。ITGB2高表达也与年龄较大（P=0.02）和CR率较低（P=0.020）相关。此外，ITGB2高表达预示着较差的OS（P=0.028）。