Horváth Csaba Memorial Laboratory of Bioseparation Sciences, Doctoral School of Molecular Medicine, Research Center for Molecular Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
Research Institute of Biomolecular and Chemical Engineering, University of Pannonia, Veszprem, Hungary.
Adv Exp Med Biol. 2021;1336:129-137. doi: 10.1007/978-3-030-77252-9_7.
Glycomics has a growing interest in the biopharmaceutical industry and biomedical research requiring new high-performance and high-sensitivity bioanalytical tools. Analysis of N-glycosylation is very important during the development of protein therapeutics and it also plays a key role in biomarker discovery. The most frequently used glycoanalytical methods are capillary electrophoresis, liquid chromatography, and mass spectrometry. In this chapter, the capillary electrophoresis-based N-linked carbohydrate analysis methods are conferred with emphasis on its use in the biopharmaceutical and biomedical fields.
糖组学在生物制药行业和需要新型高性能和高灵敏度生物分析工具的生物医学研究中越来越受到关注。在蛋白治疗药物的开发过程中，N-糖基化分析非常重要，它在生物标志物发现中也起着关键作用。最常用的糖分析方法是毛细管电泳、液相色谱和质谱。本章重点介绍了基于毛细管电泳的 N-连接糖分析方法在生物制药和生物医学领域的应用。
