小骨散减轻碘乙酸钠诱导的骨关节炎大鼠的疼痛和软骨损伤。
The Xiaogu San Attenuates Pain and Cartilage Damage in Rats with Monosodium Iodoacetate Induced Osteoarthritis.
机构信息
Department of Orthopedics, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530011, Guangxi Zhuang Autonomous Region, China.
Department of Acupuncture, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530011, Guangxi Zhuang Autonomous Region, China.
出版信息
Comb Chem High Throughput Screen. 2022;25(11):1914-1951. doi: 10.2174/1386207325666211008130312.
BACKGROUND
Osteoarthritis (OA) is a degenerative joint disease with an increasing incidence associated with increased life expectancy. The application of traditional Chinese medicine in the treatment of OA has become a research hotspot.
OBJECTIVE
This study investigated the effects of XGS externally applied to osteoarthritic joints and analyze its effect on pain in monosodium iodoacetate (MIA)-induced OA rats. This study also evaluates potential mechanisms behind the anti-osteoarthritic effects of XGS.
METHODS
A total of 24 Sprague Dawley rats were evenly and randomly divided into three separate groups, including the normal control (NC), OA and XGS groups. MIA (50 μL, 10 mg/mL) was injected into the left knee joints of the rats to induce OA. After 7 days, The rats of XGS group were given XGS (0.45 g) that was externally applied to the left knee joint, were fixed with gauze, and continuously administered XGS for 28 days. Morphological changes in tissues and organs were examined using H&E staining. Biochemical indicators were measured using an automatic biochemical analyzer. Inflammatory cytokines were detected using ELISA kits and immunohistochemistry. RNA-based high-throughput sequencing (RNA-seq) was performed to detect differential expression of mRNAs in normal and MIA-induced OA rats.
RESULTS
Stride of the left leg was extended in rats, matrix increased on cartilage tissue surfaces, and inflammatory cytokines were reduced when treated with XGS. RNA-seq results revealed that the PI3K-Akt signaling pathway is activated in the OA model. The qRT-PCR showed that the expression levels of Tnn, Col6a6, Igf1 and Lamb1 were up-regulated by XGS.
CONCLUSION
Altogether, this work demonstrated the potential therapeutic effects of XGS in rats with OA induced by MIA. The XGS may be considered an alternative therapy to manage OA.
背景
骨关节炎（OA）是一种退行性关节疾病，随着预期寿命的延长，其发病率不断增加。中药在 OA 治疗中的应用已成为研究热点。
目的
本研究探讨了消骨膏（XGS）外用对骨关节炎关节的影响，并分析其对碘乙酸单钠（MIA）诱导的 OA 大鼠疼痛的作用。本研究还评估了 XGS 抗骨关节炎作用的潜在机制。
方法
将 24 只 Sprague Dawley 大鼠均匀随机分为三组，分别为正常对照组（NC）、OA 组和 XGS 组。向大鼠左膝关节内注射 MIA（50 μL，10 mg/mL）诱导 OA。7 天后，XGS 组大鼠给予 XGS（0.45 g）外用左膝关节，用纱布固定，连续给药 28 天。用 H&E 染色观察组织器官的形态变化。用自动生化分析仪检测生化指标。用 ELISA 试剂盒和免疫组化检测炎症细胞因子。采用 RNA 高通量测序（RNA-seq）检测正常和 MIA 诱导的 OA 大鼠中 mRNA 的差异表达。
结果
XGS 治疗后，大鼠左腿步幅延长，软骨组织表面基质增加，炎症细胞因子减少。RNA-seq 结果显示，OA 模型中 PI3K-Akt 信号通路被激活。qRT-PCR 结果显示，XGS 上调了 Tnn、Col6a6、Igf1 和 Lamb1 的表达水平。
结论
综上所述，本研究工作表明 XGS 对 MIA 诱导的 OA 大鼠具有潜在的治疗作用。XGS 可作为治疗 OA 的一种替代疗法。
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