Golge Ozgur, Liman Turan, Kabak Bulent
Alanya Alaaddin Keykubat University, Faculty of Tourism, Department of Gastronomy and Culinary Arts, Alanya, Antalya, Turkey.
STA Food Quality Control Laboratory, Akdeniz, Mersin, Turkey.
Food Sci Anim Resour. 2021 Sep;41(5):816-825. doi: 10.5851/kosfa.2021.e38. Epub 2021 Sep 1.
This study aims to validate a fast method of simultaneous analysis of 365 LC-amenable and 142 GC-amenable pesticides in hen eggs by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and gas chromatography-tandem mass spectrometry (GC-MS/MS), respectively, operating in multiple reaction monitoring (MRM) acquisition modes. The sample preparation was based on quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction. Key method performance parameters investigated were specificity, linearity, limit of quantification (LOQ), accuracy, precision and measurement uncertainty. The method was validated at two spiking levels (10 and 50 μg/kg), and good recoveries (70%-120%) and relative standard deviations (RSDs) (≤20) were achieved for 92.9% of LC-amenable and 86.6% of GC-amenable pesticide residues. The LOQs were ≤10 μg/kg for 94.2% of LC-amenable and 92.3% of GC-amenable pesticides. The validated method was further applied to 100 egg samples from caged hens, and none of the pesticides was quantified.
本研究旨在验证一种快速方法，分别通过液相色谱 - 串联质谱法（LC-MS/MS）和气相色谱 - 串联质谱法（GC-MS/MS），在多反应监测（MRM）采集模式下，同时分析鸡蛋中365种适合LC分析和142种适合GC分析的农药。样品制备基于快速、简便、廉价、有效、耐用且安全（QuEChERS）萃取法。所研究的关键方法性能参数包括特异性、线性、定量限（LOQ）、准确度、精密度和测量不确定度。该方法在两个加标水平（10和50μg/kg）下进行了验证，对于92.9%的适合LC分析和86.6%的适合GC分析的农药残留，实现了良好的回收率（70%-120%）和相对标准偏差（RSDs）（≤20）。对于94.2%的适合LC分析和92.3%的适合GC分析的农药，定量限≤10μg/kg。经验证的方法进一步应用于100个笼养母鸡产的鸡蛋样品，未检测到任何农药的定量结果。
