Liu Saisai, Song Dan, Yuan Dongya
Engineering Research Center of Tibetan Medicine Detection Technology, Ministry of Education, School of Medicine, Xizang Minzu University, Xianyang, 712082, Shaanxi, China.
Key Laboratory for Molecular Genetic Mechanisms and Intervention Research On High Altitude Disease of Tibet Autonomous Region, School of Medicine, Xizang Minzu University, Xianyang, 712082, Shaanxi, China.
Immunol Res. 2022 Feb;70(1):33-43. doi: 10.1007/s12026-021-09235-y. Epub 2021 Oct 11.
Bergamottin is a natural furanocoumarin compound that possesses antioxidative and anti-cancer properties; however, the effect of Bergamottin on lipopolysaccharide (LPS)-induced inflammation response is unknown. In this study, we investigated the protective effects and mechanisms of Bergamottin against LPS-induced inflammatory responses.Raw264.7 cells were pre-treated with Bergamottin, then stimulated with LPS. Morphologic analysis and flow cytometry were used to measure Bergamottin-related cytotoxicity. ELISA and qPCR were performed to measure secretion and transcription activities of inflammatory cytokines. Biochemical analysis was used to determine the expression of tissues damage indicators. Western blots were used to determine protein expression, and immunofluorescence staining was used to determine the co-localization of NF-κB and RelA. Hematoxylin and eosin staining was used to show the pathological damages.Bergamottin had no cytotoxic effects on Raw264.7 cells. Pre-treatment with Bergamottin inhibited inflammatory cytokines expression and secretion induced by LPS, due to the inhibition of LPS-induced NF-κB signaling pathway activation, and improved pathological damages. These findings suggest that Bergamottin protects against LPS-induced endotoxin shock by regulating the NF-κB signaling pathway.
佛手柑内酯是一种天然呋喃香豆素化合物，具有抗氧化和抗癌特性；然而，佛手柑内酯对脂多糖（LPS）诱导的炎症反应的影响尚不清楚。在本研究中，我们调查了佛手柑内酯对LPS诱导的炎症反应的保护作用及其机制。将Raw264.7细胞用佛手柑内酯预处理，然后用LPS刺激。采用形态学分析和流式细胞术检测佛手柑内酯相关的细胞毒性。进行ELISA和qPCR检测炎症细胞因子的分泌和转录活性。采用生化分析确定组织损伤指标的表达。用蛋白质免疫印迹法确定蛋白质表达，用免疫荧光染色法确定NF-κB和RelA的共定位。用苏木精和伊红染色显示病理损伤。佛手柑内酯对Raw264.7细胞无细胞毒性作用。佛手柑内酯预处理可抑制LPS诱导的炎症细胞因子的表达和分泌，这是由于抑制了LPS诱导的NF-κB信号通路激活，并改善了病理损伤。这些发现表明，佛手柑内酯通过调节NF-κB信号通路来保护机体免受LPS诱导的内毒素休克。
