College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
J Agric Food Chem. 2021 Oct 20;69(41):12354-12367. doi: 10.1021/acs.jafc.1c02703. Epub 2021 Oct 11.
Flavonoids in grapes contribute the quality of the berry, but the flavonoid diversity and the regulatory networks underlying the variation require a further investigation. In this study, we integrated multi-omics data to systematically explore the global metabolic and transcriptional profiles in the skins and pulps of three grape cultivars. The results revealed large-scale differences involved in the flavonoid metabolic pathway. A total of 133 flavonoids, including flavone and flavone -glycosides, were identified. Beyond the visible differences of anthocyanins, there was large variation in other sub-branched flavonoids, most of which were positively correlated with anthocyanins in grapes. The expressions of most flavonoid biosynthetic genes and the major regulators were strongly consistent with the changes in flavonoids. Integrative analysis identified two novel transcription factors ( and ) and two ubiquitin proteins () as promising regulatory candidates for flavonoid biosynthesis in grapes. Further verification in various grape accessions indicated that five major genes including (), , , (), and () controlled flavonoid variation in grape berries. These findings provide valuable information for understanding the mechanism of flavonoid biosynthesis in grape berries and the further development of grape health products.
葡萄中的类黄酮影响浆果的品质，但类黄酮的多样性和调控网络的变异还需要进一步研究。在这项研究中，我们整合了多组学数据，系统地研究了三个葡萄品种的果皮和果肉的全局代谢和转录谱。结果揭示了参与类黄酮代谢途径的大规模差异。共鉴定出 133 种类黄酮，包括黄酮和黄酮糖苷。除了花青素的明显差异外，其他亚分支类黄酮也存在很大的差异，其中大多数与葡萄中的花青素呈正相关。大多数类黄酮生物合成基因的表达和主要调控因子与类黄酮的变化强烈一致。综合分析确定了两个新的转录因子（和）和两个泛素蛋白（）作为葡萄中类黄酮生物合成有前途的调控候选物。在各种葡萄品种中的进一步验证表明，包括（）、（）、（）、（）和（）在内的五个主要基因控制着葡萄浆果中类黄酮的变异。这些发现为了解葡萄浆果中类黄酮生物合成的机制以及葡萄保健品的进一步开发提供了有价值的信息。
