Université de Reims Champagne-Ardenne, ESCAPE EA 7510, Centre Hospitalier de Reims et Université de Reims Champagne-Ardenne, Reims, France.
Université de Reims Champagne-Ardenne, CNRS, ICMR 7312, Reims, France.
Antimicrob Agents Chemother. 2022 Jan 18;66(1):e0109821. doi: 10.1128/AAC.01098-21. Epub 2021 Oct 11.
Toxoplasmosis is a worldwide parasitosis that affects one-third of the population. People at risk, such as immunocompromised patients (AIDS, chemotherapy treatment) or fetuses (maternal-fetal transmission) can develop severe forms of the disease. The antiparasitic activity of extracts of different polarities (-heptane, MeOH, MeOH/HO) of 10 tree species endemic to temperate regions was investigated against Toxoplasma gondii infection . Our results showed that the -heptane extract of the black alder () exhibited a significant antiparasitic activity without any cytotoxicity at the tested concentrations, with an IC of up to 25.08 μg/mL and a selectivity index higher than 3.99. The chemical profiling of this extract revealed triterpenes as major constituents. The ability of commercially available triterpene (betulin, betulinic acid, and betulone) to inhibit the growth of T. gondii was evaluated and showed growth inhibition rates of 44%, 49%, and 99% at 10 μM, respectively.
弓形虫病是一种全球性寄生虫病，影响全球三分之一的人口。处于危险中的人，如免疫功能低下的患者（艾滋病、化疗治疗）或胎儿（母婴传播），可能会出现严重的疾病形式。本研究调查了 10 种温带地区特有树种的不同极性（-庚烷、甲醇、甲醇/水）提取物对刚地弓形虫感染的抗寄生虫活性。结果表明，黑桤木（）的 -庚烷提取物在测试浓度下无细胞毒性，表现出显著的抗寄生虫活性，IC 高达 25.08μg/mL，选择性指数高于 3.99。该提取物的化学成分分析显示三萜类化合物为主要成分。评估了市售三萜（白桦脂醇、白桦脂酸和桦木酮）抑制刚地弓形虫生长的能力，结果表明，在 10μM 时，它们的生长抑制率分别为 44%、49%和 99%。
