College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Shanghai Aquatic Products Processing and Storage Engineering Technology Research Center, Shanghai 201306, China; National Experimental Teaching Demonstration Center for Food Science and Engineering (Shanghai Ocean University), Shanghai 201306, China.
Ultrason Sonochem. 2021 Nov;79:105787. doi: 10.1016/j.ultsonch.2021.105787. Epub 2021 Oct 8.
The effects of multi-frequency ultrasound on surface decontamination and structural characteristics of large yellow croaker (Pseudosciaena crocea) during refrigerated storage were evaluated. The results of total viable counts (TVCs) and psychrophilic bacteria counts (PBCs) demonstrated that multi-frequency ultrasound retarded the growth of microorganisms. The bacteriostatic effect was positively correlated with the increase of ultrasound frequencies. However, compared with triple-frequency ultrasound (TUS, 20/28/40 kHz) treatment, dual-frequency ultrasound (DUS, 20/28 kHz) treatment had higher water-holding capacity (WHC) and immobilized water content, better texture characteristics, lower pH and total volatile basic nitrogen (TVB-N). Through the results of myofibrillar fragmentation index (MFI), intrinsic fluorescence intensity (IFI) and atomic force microscope (AFM), multi-frequency ultrasound could effectively stabilize the myofibrillar protein structure of refrigerated large yellow croaker, which could maintain better texture characteristics. The effects of DUS were the most significant. Therefore, multi-frequency ultrasound treatment could inhibit the growth of microorganisms and improve the structural characteristics of large yellow croaker during refrigerated storage.
研究了多频超声波对冷藏大黄鱼表面除菌效果及结构特性的影响。总活菌数（TVC）和嗜冷菌计数（PBC）的结果表明，多频超声波能抑制微生物的生长。抑菌效果与超声频率的增加呈正相关。然而，与三频超声（TUS，20/28/40 kHz）处理相比，双频超声（DUS，20/28 kHz）处理具有更高的持水能力（WHC）和结合水含量、更好的质构特性、更低的 pH 值和总挥发性碱性氮（TVB-N）。通过肌原纤维碎片化指数（MFI）、内源荧光强度（IFI）和原子力显微镜（AFM）的结果可知，多频超声波能有效稳定冷藏大黄鱼肌原纤维蛋白结构，保持更好的质构特性。DUS 的效果最为显著。因此，多频超声处理能抑制冷藏过程中大黄鱼微生物的生长，改善其结构特性。
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