College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
Shanghai Aquatic Products Processing and Storage Engineering Technology Research Center, Shanghai, China.
J Food Sci. 2021 Feb;86(2):484-494. doi: 10.1111/1750-3841.15596. Epub 2021 Jan 15.
This study assessed the effects of ε-polylysine hydrochloride (ε-PLH) and rosemary extract (RE) combined with slightly acidic electrolyzed water (SAEW) on the shelf life of obscure pufferfish (Takifugu obscurus) during refrigerated storage at (4 ± 1 °C). The samples were first dipped in SAEW and then treated with sterilized water (CK) and other solutions (0.2% RE [RE], 0.3% ε-PLH [PLH], and 0.2% RE + 0.3% ε-PLH [RE+PLH], respectively). Afterwards, microbiological, physicochemical, water migration, and sensory attributes were periodically assessed. The results showed that compared with CK and RE groups, ε-PLH treatment could effectively inhibit microbial growth and delay the increase in total volatile base nitrogen (TVB-N). In addition, microbial indexes, TVB-N, thiobarbituric acid, and metmyoglobin value in the samples treated with RE+ε-PLH were lower than those with ε-PLH or RE alone during storage. Meanwhile, samples treated with RE+ε-PLH exhibited the best sensory properties and no effect on color parameters. Based on the results of microbiological analysis, the shelf life of obscure pufferfish treated with RE+ε-PLH was 14 days during refrigerated storage at (4 ± 1 °C). PRACTICAL APPLICATION: In our study, the combined application of ε-PLH, RE, and SAEW was effective to extend the shelf life of obscure pufferfish. Based on the results of total viable counts, the RE+PLH group had a shelf life of approximately 14 days. Accordingly, bio-preservatives combined with SAEW technology have a broad application potential in the storage of obscure pufferfish.
本研究评估了ε-聚赖氨酸盐酸盐（ε-PLH）和迷迭香提取物（RE）与微酸性电解水（SAEW）结合对冷藏（4±1°C）条件下暗纹东方鲀货架期的影响。样品首先浸入 SAEW 中，然后用无菌水（CK）和其他溶液（0.2%RE[RE]、0.3%ε-PLH[PLH]和 0.2%RE+0.3%ε-PLH[RE+PLH]）处理。随后定期评估微生物学、理化、水分迁移和感官属性。结果表明，与 CK 和 RE 组相比，ε-PLH 处理能有效抑制微生物生长，延缓总挥发性碱基氮（TVB-N）的增加。此外，在贮藏过程中，RE+ε-PLH 处理的样品中微生物指标、TVB-N、硫代巴比妥酸和高铁肌红蛋白值均低于 ε-PLH 或 RE 单独处理的样品。同时，RE+ε-PLH 处理的样品感官特性最佳，对颜色参数无影响。基于微生物分析的结果，在（4±1°C）冷藏条件下，用 RE+ε-PLH 处理的暗纹东方鲀的货架期为 14 天。实际应用：在本研究中，ε-PLH、RE 和 SAEW 的联合应用有效地延长了暗纹东方鲀的货架期。基于总活菌计数的结果，RE+PLH 组的货架期约为 14 天。因此，生物防腐剂与 SAEW 技术的结合在暗纹东方鲀的贮藏中有广阔的应用潜力。
