Den Besten P K
J Dent Res. 1986 Oct;65(10):1272-7. doi: 10.1177/00220345860650101401.
Enamel maturation consists of a loss of the early secreted matrix proteins and an increase in mineralization. This study investigated the changes in enamel proteins of the rat incisor, caused by the ingestion of fluoride at various stages of enamel formation. Rats were given 0, 10, 25, 50, or 100 ppm fluoride in drinking water for five weeks. Changes in the protein composition of the secretory, early-maturation, and late-maturation enamel were investigated by means of gel filtration chromatography and polyacrylamide gel electrophoresis. No differences were found between fluorosed and control enamel proteins in secretory enamel. In fluorosed early-maturation enamel, amelogenins were retained in larger quantities than in control enamel in animals ingesting 25 ppm fluoride or greater. At the late-maturation stage of enamel formation, only enamel from animals ingesting 100 ppm fluoride in drinking water contained more protein, when compared with control enamel. This study suggests that fluoride ingestion levels resulting in enamel fluorosis inhibit the mechanisms involved in the removal of proteins during enamel maturation.
釉质成熟包括早期分泌的基质蛋白的丢失和矿化程度的增加。本研究调查了在釉质形成的各个阶段摄入氟化物对大鼠切牙釉质蛋白的影响。给大鼠饮用含0、10、25、50或100 ppm氟化物的水，持续五周。通过凝胶过滤色谱法和聚丙烯酰胺凝胶电泳研究分泌期、早期成熟期和晚期成熟期釉质的蛋白质组成变化。在分泌期釉质中，氟斑釉质蛋白与对照釉质蛋白之间未发现差异。在摄入25 ppm或更高浓度氟化物的动物中，氟斑早期成熟釉质中的釉原蛋白保留量比对照釉质中的更多。在釉质形成的晚期成熟阶段，与对照釉质相比，只有饮用含100 ppm氟化物水的动物的釉质含有更多蛋白质。本研究表明，导致釉质氟斑的氟化物摄入水平会抑制釉质成熟过程中蛋白质去除所涉及的机制。
