School of Medical Science, Griffith University, Southport, QLD 4222, Australia.
Australian Rivers Institute, School of Environment and Science, Griffith University, Brisbane, QLD 4111, Australia.
Int J Mol Sci. 2021 Sep 23;22(19):10223. doi: 10.3390/ijms221910223.
Linoleic acid (LA) is an essential polyunsaturated fatty acid (PUFA) that is required for foetal growth and development. Excess intake of LA can be detrimental for metabolic health due to its pro-inflammatory properties; however, the effect of a diet high in LA on offspring metabolites is unknown. In this study, we aimed to determine the role of maternal or postnatal high linoleic acid (HLA) diet on plasma metabolites in adult offspring.
Female Wistar Kyoto (WKY) rats were fed with either low LA (LLA) or HLA diet for 10 weeks prior to conception and during gestation/lactation. Offspring were weaned at postnatal day 25 (PN25), treated with either LLA or HLA diets and sacrificed at PN180. Metabolite analysis was performed in plasma samples using Nuclear Magnetic Resonance.
Maternal and postnatal HLA diet did not alter plasma metabolites in male and female adult offspring. There was no specific clustering among different treatment groups as demonstrated by principal component analysis. Interestingly, there was clustering among male and female offspring independent of maternal and postnatal dietary intervention. Lysine was higher in female offspring, while 3-hydroxybutyric acid and acetic acid were significantly higher in male offspring.
In summary, maternal or postnatal HLA diet did not alter the plasma metabolites in the adult rat offspring; however, differences in metabolites between male and female offspring occurred independently of dietary intervention.
亚油酸（LA）是一种必需的多不饱和脂肪酸（PUFA），对于胎儿的生长和发育是必需的。由于其具有促炎特性，LA 摄入过量可能对代谢健康有害；然而，高 LA 饮食对后代代谢物的影响尚不清楚。在这项研究中，我们旨在确定母体或产后高亚油酸（HLA）饮食对成年后代血浆代谢物的作用。
雌性 Wistar Kyoto（WKY）大鼠在受孕前和妊娠期/哺乳期前 10 周分别用低 LA（LLA）或 HLA 饮食喂养。后代在出生后第 25 天（PN25）断奶，用 LLA 或 HLA 饮食处理，并在 PN180 时处死。使用核磁共振技术在血浆样本中进行代谢物分析。
母体和产后 HLA 饮食未改变雄性和雌性成年后代的血浆代谢物。主成分分析显示，不同处理组之间没有特定的聚类。有趣的是，无论母体和产后饮食干预如何，雄性和雌性后代之间都存在聚类。赖氨酸在雌性后代中较高，而 3-羟基丁酸和乙酸在雄性后代中显著较高。
总之，母体或产后 HLA 饮食并未改变成年大鼠后代的血浆代谢物；然而，雄性和雌性后代之间的代谢物差异独立于饮食干预。
