Koeppen A H, Mitzen E J, Hans M B, Barron K D
Neurology. 1986 Nov;36(11):1478-88. doi: 10.1212/wnl.36.11.1478.
Brain tissue was obtained promptly after death from a patient with autosomal dominant olivopontocerebellar atrophy and studied by immunocytochemistry and a Golgi technique. Antiglutamic acid decarboxylase showed severe loss of Purkinje cells and their terminals in the dentate nucleus. Stains for neuron-specific enolase (NSE) and microtubule-associated proteins (MAP) confirmed the integrity of the dentate nucleus. Basket and stellate cells revealed secondary changes, but Golgi neurons were intact. Methods for NSE and MAP disclosed dendritic alterations and loss of neurons in the basis pontis and inferior olivary nuclei. Golgi impregnation of Purkinje cells showed loss of major dendrites, paucity of spiny branchlets, and axonal expansions.
从一名患有常染色体显性遗传性橄榄体脑桥小脑萎缩的患者死亡后迅速获取脑组织，并通过免疫细胞化学和高尔基技术进行研究。抗谷氨酸脱羧酶显示浦肯野细胞及其在齿状核中的终末严重缺失。神经元特异性烯醇化酶（NSE）和微管相关蛋白（MAP）染色证实了齿状核的完整性。篮状细胞和星形细胞显示出继发性改变，但高尔基神经元完好无损。NSE和MAP的检测方法揭示了脑桥基底部和下橄榄核中的树突改变和神经元缺失。浦肯野细胞的高尔基浸染显示主要树突缺失、棘状小分支稀少以及轴突扩张。
