State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Kaifeng 475004, China.
Int J Mol Sci. 2021 Oct 1;22(19):10668. doi: 10.3390/ijms221910668.
Chloroplasts of higher plants are semi-autonomous organelles that perform photosynthesis and produce hormones and metabolites. They play crucial roles in plant growth and development. Although many seedling-lethal nuclear genes or regulators required for chloroplast development have been characterized, the understanding of chloroplast development is still limited. Using a genetic screen, we isolated a mutant named ell1, with etiolated leaves and a seedling-lethal phenotype. Analysis by BN-PAGE and transmission electron microscopy revealed drastic morphological defects of chloroplasts in ell1 mutants. Genetic mapping of the mutant gene revealed a single mutation (G-to-A) at the 5' splice site of intron 5 in , resulting in an exon skipping in , indicating that this mutation in is responsible for the observed phenotype, which was further confirmed by genetic analysis. The incorrectly spliced failed to mediate the splicing of intron. Moreover, the quantitative analysis suggested that may participate in chloroplast transcription to regulate the development of chloroplast. Taken together, these findings improve our understanding of the protein and shed new light on the regulation of chloroplast development in maize.
高等植物的叶绿体是半自主的细胞器，能够进行光合作用并产生激素和代谢物。它们在植物的生长和发育中起着至关重要的作用。尽管已经鉴定出许多对叶绿体发育所必需的核基因或调控因子，但对叶绿体发育的理解仍然有限。通过遗传筛选，我们分离到一个名为 ell1 的突变体，其表现为黄化叶片和幼苗致死表型。BN-PAGE 和透射电子显微镜分析显示 ell1 突变体的叶绿体在形态上存在严重缺陷。突变基因的遗传定位显示，突变发生在 5' 内含子 5 的剪接位点，导致 外显子跳跃，表明该突变是造成观察到的表型的原因，这一结果通过遗传分析进一步得到了证实。错误剪接的 无法介导 内含子的剪接。此外，定量分析表明 可能参与叶绿体转录，从而调控叶绿体的发育。总之，这些发现提高了我们对 蛋白的认识，并为玉米中叶绿体发育的调控提供了新的线索。
