Laboratory of Veterinary Pharmacology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan.
Department of Pharmacology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Elqaliobiya, Egypt.
Int J Mol Sci. 2021 Oct 5;22(19):10763. doi: 10.3390/ijms221910763.
Various pathogens, such as Ebola virus, Marburg virus, Nipah virus, Hendra virus, Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV), Middle East Respiratory Syndrome Coronavirus (MERS-CoV), and SARS-CoV-2, are threatening human health worldwide. The natural hosts of these pathogens are thought to be bats. The rousette bat, a megabat, is thought to be a natural reservoir of filoviruses, including Ebola and Marburg viruses. Additionally, the rousette bat showed a transient infection in the experimental inoculation of SARS-CoV-2. In the current study, we established and characterized intestinal organoids from Leschenault's rousette, . The established organoids successfully recapitulated the characteristics of intestinal epithelial structure and morphology, and the appropriate supplements necessary for long-term stable culture were identified. The organoid showed susceptibility to Pteropine orthoreovirus (PRV) but not to SARS-CoV-2 in experimental inoculation. This is the first report of the establishment of an expandable organoid culture system of the rousette bat intestinal organoid and its sensitivity to bat-associated viruses, PRV and SARS-CoV-2. This organoid is a useful tool for the elucidation of tolerance mechanisms of the emerging rousette bat-associated viruses such as Ebola and Marburg virus.
各种病原体，如埃博拉病毒、马尔堡病毒、尼帕病毒、亨德拉病毒、严重急性呼吸综合征冠状病毒（SARS-CoV）、中东呼吸综合征冠状病毒（MERS-CoV）和 SARS-CoV-2，正在威胁着全球人类的健康。这些病原体的自然宿主被认为是蝙蝠。果蝠是一种大型蝙蝠，被认为是包括埃博拉病毒和马尔堡病毒在内的丝状病毒的天然宿主。此外，果蝠在 SARS-CoV-2 的实验接种中表现出一过性感染。在本研究中，我们从 Leschenault 的果蝠中建立并鉴定了肠道类器官。所建立的类器官成功地重现了肠道上皮结构和形态的特征，并确定了长期稳定培养所需的适当补充剂。类器官在实验接种中对 Pteropine orthoreovirus（PRV）表现出敏感性，但对 SARS-CoV-2 不敏感。这是首例报道可扩增的 Rousettus 蝙蝠肠道类器官培养系统及其对蝙蝠相关病毒（PRV 和 SARS-CoV-2）的敏感性。该类器官是阐明新兴 Rousettus 蝙蝠相关病毒（如埃博拉病毒和马尔堡病毒）的耐受机制的有用工具。
