• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

有助于理解甲基汞中毒中脑与外周的串扰：神经毒性与细胞外囊泡。

Contributing to Understand the Crosstalk between Brain and Periphery in Methylmercury Intoxication: Neurotoxicity and Extracellular Vesicles.

机构信息

Laboratório de Farmacologia Molecular, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém 66075-110, Brazil.

Department of Pharmacology, University of Oxford, Oxford OX1 3QT, UK.

出版信息

Int J Mol Sci. 2021 Oct 7;22(19):10855. doi: 10.3390/ijms221910855.

DOI:10.3390/ijms221910855
PMID:34639196
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8509412/
Abstract

Human exposure to methylmercury (MeHg) is currently high in regions such as the Amazon. Understanding the molecular changes associated with MeHg-induced neurotoxicity and the crosstalk with the periphery is essential to support early diagnoses. This work aimed to evaluate cellular and molecular changes associated with behavioral alterations in MeHg acute exposure and the possible changes in extracellular vesicles (EVs) number and S100β content. Adults male Wistar rats were orally treated with 5 mg/kg for four days. Behavioral performance, molecular and histological changes in the cerebellum, and plasma EVs were assessed. MeHg-intoxicated animals performed significantly worse in behavioral tests. MeHg increased the number of GFAP+ cells and GFAP and S100β mRNA expression in the cerebellum but no change in NeuN+ or IBA-1+ cells number was detected. The number of exosomes isolated from plasma were decreased by the metal. S100B mRNA was detected in circulating plasma EVs cargo in MeHg exposure. Though preliminary, our results suggest astrocytic reactivity is displaying a protective role once there was no neuronal death. Interestingly, the reduction in exosomes number could be a new mechanism associated with MeHg-induced neurotoxicity and plasma EVs could represent a source of future biomarkers in MeHg intoxication.

摘要

人类暴露于甲基汞（MeHg）的情况目前在亚马逊地区等地区很高。了解与 MeHg 诱导的神经毒性相关的分子变化以及与外围的串扰对于支持早期诊断至关重要。这项工作旨在评估与 MeHg 急性暴露相关的行为改变以及细胞外囊泡（EVs）数量和 S100β 含量可能发生的变化相关的细胞和分子变化。成年雄性 Wistar 大鼠连续 4 天经口给予 5mg/kg MeHg。评估行为表现、小脑的分子和组织学变化以及血浆 EVs。MeHg 中毒动物在行为测试中表现明显更差。MeHg 增加了小脑内 GFAP+细胞和 GFAP 和 S100β mRNA 的表达，但未检测到 NeuN+或 IBA-1+细胞数量的变化。从血浆中分离的外泌体数量减少。MeHg 暴露时，S100B mRNA 存在于循环血浆 EVs 载物中。虽然初步，但我们的结果表明，星形胶质细胞反应显示出一种保护作用，因为没有神经元死亡。有趣的是，外泌体数量的减少可能是与 MeHg 诱导的神经毒性相关的新机制，而血浆 EVs 可能代表 MeHg 中毒中未来生物标志物的来源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6bd/8509412/f9a5de334bd2/ijms-22-10855-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6bd/8509412/eaed245d0df7/ijms-22-10855-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6bd/8509412/e854f95c5bbf/ijms-22-10855-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6bd/8509412/7828148399f0/ijms-22-10855-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6bd/8509412/d2bbefa9ca98/ijms-22-10855-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6bd/8509412/f80f22781aca/ijms-22-10855-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6bd/8509412/f9a5de334bd2/ijms-22-10855-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6bd/8509412/eaed245d0df7/ijms-22-10855-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6bd/8509412/e854f95c5bbf/ijms-22-10855-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6bd/8509412/7828148399f0/ijms-22-10855-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6bd/8509412/d2bbefa9ca98/ijms-22-10855-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6bd/8509412/f80f22781aca/ijms-22-10855-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6bd/8509412/f9a5de334bd2/ijms-22-10855-g006.jpg

相似文献

1
Contributing to Understand the Crosstalk between Brain and Periphery in Methylmercury Intoxication: Neurotoxicity and Extracellular Vesicles.有助于理解甲基汞中毒中脑与外周的串扰：神经毒性与细胞外囊泡。
Int J Mol Sci. 2021 Oct 7;22(19):10855. doi: 10.3390/ijms221910855.
2
Delayed neurochemical effects of prenatal exposure to MeHg in the cerebellum of developing rats.产前暴露于甲基汞对发育中大鼠小脑的延迟神经化学效应。
Toxicol Lett. 2018 Mar 1;284:161-169. doi: 10.1016/j.toxlet.2017.12.006. Epub 2017 Dec 16.
3
Unravelling motor behaviour hallmarks in intoxicated adolescents: methylmercury subtoxic-dose exposure and binge ethanol intake paradigm in rats.解析中毒青少年的运动行为特征：甲基汞亚毒性剂量暴露和大鼠 binge 乙醇摄入范式。
Environ Sci Pollut Res Int. 2018 Aug;25(22):21937-21948. doi: 10.1007/s11356-018-2235-4. Epub 2018 May 24.
4
Protective effect of Bacopa monniera on methyl mercury-induced oxidative stress in cerebellum of rats.印度蔊菜对甲基汞诱导的大鼠小脑氧化应激的保护作用。
Cell Mol Neurobiol. 2012 Aug;32(6):979-87. doi: 10.1007/s10571-012-9813-7. Epub 2012 Feb 26.
5
Prenatal low-dose methylmercury exposure impairs neurite outgrowth and synaptic protein expression and suppresses TrkA pathway activity and eEF1A1 expression in the rat cerebellum.产前低剂量甲基汞暴露会损害大鼠小脑的神经突生长和突触蛋白表达，并抑制TrkA通路活性和eEF1A1表达。
Toxicol Appl Pharmacol. 2016 May 1;298:1-8. doi: 10.1016/j.taap.2016.03.002. Epub 2016 Mar 7.
6
Neuroprotective Effect of Portulaca oleraceae Ethanolic Extract Ameliorates Methylmercury Induced Cognitive Dysfunction and Oxidative Stress in Cerebellum and Cortex of Rat Brain.马齿苋乙醇提取物的神经保护作用改善甲基汞诱导的大鼠脑小脑和皮质认知功能障碍及氧化应激。
Biol Trace Elem Res. 2016 Jul;172(1):155-165. doi: 10.1007/s12011-015-0546-6. Epub 2015 Nov 12.
7
Revisiting Astrocytic Roles in Methylmercury Intoxication.重新探讨星形胶质细胞在甲基汞中毒中的作用。
Mol Neurobiol. 2021 Sep;58(9):4293-4308. doi: 10.1007/s12035-021-02420-y. Epub 2021 May 14.
8
Cellular and Molecular Mechanisms Mediating Methylmercury Neurotoxicity and Neuroinflammation.介导甲基汞神经毒性和神经炎症的细胞和分子机制。
Int J Mol Sci. 2021 Mar 18;22(6):3101. doi: 10.3390/ijms22063101.
9
Hepatic and neurobiological effects of foetal and breastfeeding and adulthood exposure to methylmercury in Wistar rats.在 Wistar 大鼠中，胎儿期、哺乳期和成年期暴露于甲基汞对肝脏和神经生物学的影响。
Chemosphere. 2020 Apr;244:125400. doi: 10.1016/j.chemosphere.2019.125400. Epub 2019 Nov 26.
10
Methylmercury exposure develops atherosclerotic risk factors in the aorta and programmed cell death in the cerebellum: ameliorative action of Celastrus paniculatus ethanolic extract in male Wistar rats.甲基汞暴露会在主动脉形成动脉粥样硬化风险因素，并在小脑引发程序性细胞死亡：杠柳乙醇提取物对雄性 Wistar 大鼠的改善作用。
Environ Sci Pollut Res Int. 2018 Oct;25(30):30212-30223. doi: 10.1007/s11356-018-3031-x. Epub 2018 Aug 28.

引用本文的文献

1
Characterization of extracellular vesicles and miRNA released by cerebral organoids.脑类器官释放的细胞外囊泡和微小RNA的表征
Curr Res Toxicol. 2025 Mar 8;8:100229. doi: 10.1016/j.crtox.2025.100229. eCollection 2025.
2
Toxicogenomics and Molecular Markers in Pollution.毒理基因组学与污染的分子标志物
Int J Mol Sci. 2022 Jul 27;23(15):8280. doi: 10.3390/ijms23158280.

本文引用的文献

1
Plasticity of microglia.小胶质细胞的可塑性。
Biol Rev Camb Philos Soc. 2022 Feb;97(1):217-250. doi: 10.1111/brv.12797. Epub 2021 Sep 21.
2
Eating in the Amazon: Nutritional Status of the Riverine Populations and Possible Nudge Interventions.亚马逊地区的饮食：河边居民的营养状况及可能的助推干预措施
Foods. 2021 May 6;10(5):1015. doi: 10.3390/foods10051015.
3
Salivary biomarkers and neuropsychological outcomes: A non-invasive approach to investigate pollutants-associated neurotoxicity and its effects on cognition in vulnerable populations.
唾液生物标志物与神经心理学结果：一种非侵入性方法，用于研究污染物相关神经毒性及其对易感人群认知的影响。
Environ Res. 2021 Sep;200:111432. doi: 10.1016/j.envres.2021.111432. Epub 2021 May 29.
4
Revisiting Astrocytic Roles in Methylmercury Intoxication.重新探讨星形胶质细胞在甲基汞中毒中的作用。
Mol Neurobiol. 2021 Sep;58(9):4293-4308. doi: 10.1007/s12035-021-02420-y. Epub 2021 May 14.
5
Astrocyte-derived extracellular vesicles: A double-edged sword in central nervous system disorders.星形胶质细胞衍生的细胞外囊泡：中枢神经系统疾病中的双刃剑。
Neurosci Biobehav Rev. 2021 Jun;125:148-159. doi: 10.1016/j.neubiorev.2021.02.027. Epub 2021 Feb 21.
6
Extracellular Vesicles in Neuroinflammation.神经炎症中的细胞外囊泡
Front Cell Dev Biol. 2021 Jan 21;8:623039. doi: 10.3389/fcell.2020.623039. eCollection 2020.
7
Human neurotoxicity of mercury in the Amazon: A scoping review with insights and critical considerations.亚马逊地区汞对人类的神经毒性：一项范围界定审查，提供了深入见解和关键考虑因素。
Ecotoxicol Environ Saf. 2021 Jan 15;208:111686. doi: 10.1016/j.ecoenv.2020.111686. Epub 2020 Dec 8.
8
Mercury: What can we learn from the Amazon?汞：我们能从亚马逊学到什么？
Environ Int. 2021 Jan;146:106223. doi: 10.1016/j.envint.2020.106223. Epub 2020 Oct 27.
9
AMPA receptor contribution to methylmercury-mediated alteration of intracellular Ca concentration in human induced pluripotent stem cell motor neurons.AMPA 受体在甲基汞致人诱导多能干细胞运动神经元细胞内 Ca 浓度改变中的作用。
Neurotoxicology. 2020 Dec;81:116-126. doi: 10.1016/j.neuro.2020.09.037. Epub 2020 Sep 28.
10
Astroglia-specific contributions to the regulation of synapses, cognition and behaviour.星形胶质细胞对突触、认知和行为调节的特异性贡献。
Neurosci Biobehav Rev. 2020 Nov;118:331-357. doi: 10.1016/j.neubiorev.2020.07.039. Epub 2020 Aug 6.