鲤鱼脱氧血红蛋白从紧张态到松弛态转变过程中的局部铁位点结构：一项X射线吸收近边结构（XANES）研究。
Local Fe site structure in the tense-to-relaxed transition in carp deoxyhemoglobin: a XANES (x-ray absorption near edge structure) study.
作者信息
Bianconi A, Congiu-Castellano A, Dell'Ariccia M, Giovannelli A, Morante S, Burattini E, Durham P J
出版信息
Proc Natl Acad Sci U S A. 1986 Oct;83(20):7736-40. doi: 10.1073/pnas.83.20.7736.
Abstract
The Fe-site structure variation in the transition from the low-affinity tense (T) quaternary structure to the high-affinity relaxed (R) structure in carp deoxyhemoglobin was studied by analysis of multiple scattering resonances in the XANES (x-ray absorption near edge structure) spectra. High signal-to-noise XANES spectra were measured at the Frascati "wiggler" synchrotron radiation facility. We find that the forces on the Fe active site due to the change of quaternary protein conformation do not induce variations greater than 0.01 A in interatomic Fe-N distances, variations greater than 0.1 A in the Fe displacement toward the heme plane, or the "doming" of the heme. The relevance of these results to the mechanism of protein control of ligand binding is discussed.
摘要
通过分析X射线吸收近边结构（XANES）光谱中的多重散射共振，研究了鲤鱼脱氧血红蛋白从低亲和力紧张（T）四级结构向高亲和力松弛（R）结构转变过程中的铁位点结构变化。在弗拉斯卡蒂“摆动器”同步辐射设施上测量了高信噪比的XANES光谱。我们发现，由于四级蛋白质构象变化而施加在铁活性位点上的力，不会导致铁-氮原子间距离的变化大于0.01埃，铁向血红素平面位移的变化大于0.1埃，也不会导致血红素的“隆起”。讨论了这些结果与蛋白质控制配体结合机制的相关性。
相似文献
1
Local Fe site structure in the tense-to-relaxed transition in carp deoxyhemoglobin: a XANES (x-ray absorption near edge structure) study.鲤鱼脱氧血红蛋白从紧张态到松弛态转变过程中的局部铁位点结构：一项X射线吸收近边结构（XANES）研究。
Proc Natl Acad Sci U S A. 1986 Oct;83(20):7736-40. doi: 10.1073/pnas.83.20.7736.
2
XANES study of iron displacement in the haem of myoglobin.
FEBS Lett. 1984 Dec 3;178(1):165-70. doi: 10.1016/0014-5793(84)81263-6.
3
A high-resolution proton nuclear-magnetic-resonance investigation of carp hemoglobin. Conformational differences between carp and human normal adult hemoglobins in solution.鲤鱼血红蛋白的高分辨率质子核磁共振研究。溶液中鲤鱼与人类正常成人血红蛋白的构象差异。
Eur J Biochem. 1984 Jun 1;141(2):255-9. doi: 10.1111/j.1432-1033.1984.tb08185.x.
4
Oxygen bonding in human hemoglobin and its isolated subunits: a XANES study.
Biochem Biophys Res Commun. 1987 Aug 31;147(1):31-8. doi: 10.1016/s0006-291x(87)80083-9.
5
Changes in Fe site structure from fetal to adult hemoglobin probed by XANES.
Biochim Biophys Acta. 1985 Sep 20;831(1):120-4. doi: 10.1016/0167-4838(85)90158-x.
6
Movement of Fe with respect to the heme plane in the R-T transition of carp hemoglobin. An extended x-ray absorption fine structure study.
J Biol Chem. 1986 May 5;261(13):5689-92.
7
On the allosteric transition between the structures of high and low ligand affinity in carp hemoglobin.鲤鱼血红蛋白中高、低配体亲和力结构之间的变构转变
Biochim Biophys Acta. 1976 Oct 28;446(2):547-53. doi: 10.1016/0005-2795(76)90022-2.
8
X-ray absorption spectroscopy of hemes and hemeproteins in solution: multiple scattering analysis.溶液中血红素和血红蛋白的X射线吸收光谱：多重散射分析
Inorg Chem. 2008 Nov 3;47(21):9905-18. doi: 10.1021/ic800982a. Epub 2008 Oct 7.
9
Structural states and transitions of carp hemoglobin.鲤鱼血红蛋白的结构状态与转变
J Biol Chem. 1975 Sep 25;250(18):7239-44.
10
Increase of the Fe effective charge in hemoproteins during oxygenation process.氧合过程中血红素蛋白中铁有效电荷的增加。
Biochem Biophys Res Commun. 1985 Aug 30;131(1):98-102. doi: 10.1016/0006-291x(85)91775-9.
引用本文的文献
1
Aggregates Sealed by Ions.离子封闭的集料。
Methods Mol Biol. 2022;2340:309-341. doi: 10.1007/978-1-0716-1546-1_14.
2
X-ray absorption spectroscopic investigation of the electronic structure differences in solution and crystalline oxyhemoglobin.X 射线吸收光谱研究溶液和结晶氧合血红蛋白中电子结构的差异。
Proc Natl Acad Sci U S A. 2013 Oct 8;110(41):16333-8. doi: 10.1073/pnas.1315734110. Epub 2013 Sep 23.
3
An XAS study of the sulfur environment in human neuromelanin and its synthetic analogs.一项 XAS 研究：人神经黑素及其合成类似物中的硫环境。
Eur Biophys J. 2010 May;39(6):959-70. doi: 10.1007/s00249-009-0462-9. Epub 2009 May 27.
4
Identifying the structure of the active sites of human recombinant prolidase.鉴定人重组脯氨酰内肽酶活性部位的结构。
Eur Biophys J. 2010 May;39(6):935-45. doi: 10.1007/s00249-009-0459-4. Epub 2009 May 5.
5
Metal binding in amyloid beta-peptides shows intra- and inter-peptide coordination modes.淀粉样β肽中的金属结合呈现出肽内和肽间的配位模式。
Eur Biophys J. 2006 Apr;35(4):340-51. doi: 10.1007/s00249-005-0041-7. Epub 2006 Jan 11.
6
An x-ray absorption spectroscopy study of the zinc environment in Langmuir-Blodgett phospholipid multilayers.对朗缪尔-布洛杰特磷脂多层膜中锌环境的X射线吸收光谱研究。
Biophys J. 2002 Dec;83(6):3507-12. doi: 10.1016/S0006-3495(02)75350-2.
本文引用的文献
1
Resonance Raman spectra of photodissociated hemoglobins: implications on cooperative mechanisms.光解血红蛋白的共振拉曼光谱：对协同机制的启示。
Biophys J. 1985 Apr;47(4):537-45. doi: 10.1016/S0006-3495(85)83948-5.
2
ON THE NATURE OF ALLOSTERIC TRANSITIONS: A PLAUSIBLE MODEL.关于别构转变的本质：一个合理的模型。
J Mol Biol. 1965 May;12:88-118. doi: 10.1016/s0022-2836(65)80285-6.
3
X-ray absorption near edge structure (XANES) for CO, CN and deoxyhaemoglobin: geometrical information.一氧化碳、氰根与脱氧血红蛋白的X射线吸收近边结构（XANES）：几何信息
EMBO J. 1983;2(9):1441-3. doi: 10.1002/j.1460-2075.1983.tb01605.x.
4
X-ray-absorption near-edge structure of 3d transition elements in tetrahedral coordination: The effect of bond-length variation.
Phys Rev B Condens Matter. 1985 Sep 15;32(6):4292-4295. doi: 10.1103/physrevb.32.4292.
5
Quaternary structures and low frequency molecular vibrations of haems of deoxy and oxyhaemoglobin studied by resonance raman scattering.通过共振拉曼散射研究脱氧血红蛋白和氧合血红蛋白血红素的四级结构及低频分子振动。
J Mol Biol. 1980 Jan 25;136(3):271-89. doi: 10.1016/0022-2836(80)90374-5.
6
Resonance Raman spectra of deoxyhemoproteins. Heme structure in relation to dioxygen binding.脱氧血红蛋白的共振拉曼光谱。与双氧结合相关的血红素结构。
Biochim Biophys Acta. 1981 Dec 29;671(2):177-83. doi: 10.1016/0005-2795(81)90132-x.
7
Transient Raman study of hemoglobin: structural dependence of the iron-histidine linkage.血红蛋白的瞬态拉曼研究：铁-组氨酸连接的结构依赖性。
Science. 1982 Dec 17;218(4578):1244-6. doi: 10.1126/science.7146910.
8
Stereochemistry of cooperative effects in fish an amphibian haemoglobins.鱼类和两栖类血红蛋白协同效应的立体化学
Nature. 1982 Sep 30;299(5882):421-6. doi: 10.1038/299421a0.
9
Resonance Raman studies of the quaternary structural change in carp deoxy hemoglobin.
FEBS Lett. 1982 Apr 19;140(2):303-6. doi: 10.1016/0014-5793(82)80919-8.
10
Resonance Raman detection of structural dynamics at the active site in hemoglobin.血红蛋白活性位点结构动力学的共振拉曼检测。
Proc Natl Acad Sci U S A. 1982 Mar;79(5):1511-4. doi: 10.1073/pnas.79.5.1511.
Zotero 插件