Tanaka T, Kobayashi T, Kida Y, Kageyama N
Gan To Kagaku Ryoho. 1986 Oct;13(10):2993-7.
The effects of heat and antitumor drugs on malignant brain tumor cell lines were studied. A human glioblastoma cell line (SKMG1) and rat malignant brain tumor cell lines (T9, EB 679 and TR 481) were used in this experiment. Five different modalities of treatment with heat and drugs were used as follows: (Group 1) exposure to heat alone at 42 degrees C for one hour; (Group 2) exposure to antitumor drug alone for one hour (ACNU 2.5 or 5 micrograms/ml, ACR 0.02 micrograms/ml and CDDP 1 microgram/ml); (Group 3) simultaneous exposure to heat at 42 degrees C and drug for one hour; (Group 4) heat at 42 degrees C given first for one hour, followed by one hour exposure to drug one hour later ("preheating"); (Group 5) drug given first for one hour, followed by one hour exposure to heat at 42 degrees C one hour later ("postheating"). After each treatment, the inhibition rate at 4 days was evaluated and compared for each group. A synergistic effect was observed in Group 3. For example, when T9 cells were exposed to ACNU and to heat at 42 degrees C at the same time for one hour, inhibition rate was 78%, while the rates for Group 1 and Group 2 were 7% and 21%, respectively. The cytotoxicity of simultaneous treatment with antitumor drugs (ACNU, ACR and CDDP) and hyperthermia at 42 degrees C was apparently superior to that of other treatment modalities.
研究了热疗和抗肿瘤药物对恶性脑肿瘤细胞系的影响。本实验使用了一种人胶质母细胞瘤细胞系（SKMG1）和大鼠恶性脑肿瘤细胞系（T9、EB 679和TR 481）。采用以下五种不同的热疗和药物治疗方式：（第1组）单独在42℃下热疗1小时；（第2组）单独使用抗肿瘤药物1小时（ACNU 2.5或5微克/毫升、ACR 0.02微克/毫升和CDDP 1微克/毫升）；（第3组）同时在42℃下热疗和使用药物1小时；（第4组）先在42℃下热疗1小时，1小时后再使用药物1小时（“预热”）；（第5组）先使用药物1小时，1小时后再在42℃下热疗1小时（“后热”）。每次治疗后，评估并比较每组在第4天的抑制率。在第3组中观察到协同效应。例如，当T9细胞同时暴露于ACNU和42℃的热疗1小时时，抑制率为78%，而第1组和第2组的抑制率分别为7%和21%。同时使用抗肿瘤药物（ACNU、ACR和CDDP）和42℃热疗的细胞毒性明显优于其他治疗方式。
