Reuss Stefan
Department of Nuclear Medicine, University Medical Center, Johannes Gutenberg-University, 55101 Mainz, Germany.
J Integr Neurosci. 2021 Sep 30;20(3):561-571. doi: 10.31083/j.jin2003060.
Location and distribution of spinal sympathetic preganglionic neurons projecting to the superior cervical ganglion were investigated in a rodent model organism for photoperiodic regulation, the Djungarian hamster (). Upon unilateral injection of Fluoro-Gold into the superior cervical ganglia, retrograde neuronal tracing demonstrated labeled neurons ipsilateral to the injection site. They were seen in spinal segments C8 to Th5 of which the segments Th1 to Th3 contained about 98% of the labeled cells. Neurons were found in the spinal cord predominantly in the intermediolateral nucleus pars principalis and pars funicularis. At the same time, the central autonomic area and the intercalated region contained only very few labeled cells. In the intermediolateral nucleus, cells often were arranged in clusters, of which several were seen in each spinal segment. Selected sections were exposed to antibodies directed against arginine-vasopressin, neuronal nitric oxide synthase, neuropeptide Y, neurotensin, oxytocin or substance P. It was found that about two-thirds of sympathetic preganglionic neurons produced the gaseous neuroactive substance nitric oxide and that few contained small amounts of neuropeptide Y. Fibers of putative supraspinal origin immunopositive for either arginine-vasopressin, neuronal nitric oxide synthase, neuropeptide Y, neurotensin, oxytocin or, in particular, substance P were found in the vicinity of labeled sympathetic preganglionic neurons. These results demonstrate the location of relay neurons for autonomic control of cranial and cardial structures and provide further knowledge on neurochemical properties of sympathetic preganglionic neurons and related structures.
在用于光周期调节的啮齿动物模型生物——黑线仓鼠中，研究了投射至上颈神经节的脊髓交感神经节前神经元的位置和分布。向上颈神经节单侧注射荧光金后，逆行神经元追踪显示注射部位同侧有标记神经元。它们见于脊髓C8至Th5节段，其中Th1至Th3节段包含约98%的标记细胞。神经元主要见于脊髓的中间外侧核的主部和索部。同时，中枢自主区和插入区仅含有极少的标记细胞。在中间外侧核中，细胞常成簇排列，每个脊髓节段可见数个细胞簇。选择的切片用针对精氨酸加压素、神经元型一氧化氮合酶、神经肽Y、神经降压素、催产素或P物质的抗体进行处理。发现约三分之二的交感神经节前神经元产生气态神经活性物质一氧化氮，少数含有少量神经肽Y。在标记的交感神经节前神经元附近发现了对精氨酸加压素、神经元型一氧化氮合酶、神经肽Y、神经降压素、催产素或特别是P物质呈免疫阳性的假定脊髓上起源的纤维。这些结果证明了用于自主控制颅部和心脏结构的中继神经元的位置，并提供了关于交感神经节前神经元及相关结构的神经化学特性的进一步知识。
