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补肝荣筋汤通过 Wnt 信号通路缓解炎症和氧化应激治疗绝经后膝骨关节炎。

Bugan Rongjin decoction alleviates inflammation and oxidative stress to treat the postmenopausal knee osteoarthritis through Wnt signaling pathway.

机构信息

Department of Orthopedics and Traumatology, Nantong TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Nantong, 226001, Jiangsu, China.

Department of Pediatrics, Nantong TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Nantong, 226001, Jiangsu, China.

出版信息

Biomed Eng Online. 2021 Oct 13;20(1):103. doi: 10.1186/s12938-021-00939-8.

DOI:10.1186/s12938-021-00939-8
PMID:34645468
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8513287/
Abstract

BACKGROUND: Traditional Chinese medicine has been found effective for the therapy of knee osteoarthritis (KOA). This study was aimed at investigating the underlying mechanism of Bugan Rongjin decoction (BGRJ) in treating the postmenopausal KOA. RESULTS: Ovariectomized rat model of KOA and LPS-induced chondrocytes were successfully constructed for in vivo and in vitro model of postmenopausal KOA. X-ray and hematoxylin-eosin (H&E) staining showed that BGRJ alleviated pathological damage of articular cartilage in OVX rats with KOA. In addition, BGRJ inhibited inflammation and oxidative stress through decreasing the levels of serum IL-6, IL-1β, TNF-α and NO and regulated Wnt signaling pathway by downregulating the expression of Wnt5a and β-catenin and upregulating the expression of Sox9 and Collagen II in cartilage tissue, detected by immunohistochemistry (IHC) and western blot analysis. Furthermore, Wnt5a silencing reduced the apoptosis of LPS-induced ADTC5 cells, which was further suppressed by the combination of downregulation of Wnt5a and BGRJ. CONCLUSIONS: In summary, BGRJ alleviates inflammation and oxidative stress to treat the postmenopausal KOA through Wnt signaling pathway.

摘要

背景：中医药已被证明对膝骨关节炎（KOA）的治疗有效。本研究旨在探讨补肝荣筋汤（BGRJ）治疗绝经后 KOA 的潜在机制。

结果：成功构建了绝经后 KOA 的体内和体外模型，即去卵巢 KOA 大鼠模型和 LPS 诱导的软骨细胞。X 射线和苏木精-伊红（H&E）染色显示，BGRJ 减轻了 OVX 大鼠 KOA 关节软骨的病理损伤。此外，BGRJ 通过降低血清 IL-6、IL-1β、TNF-α 和 NO 的水平，抑制炎症和氧化应激，通过免疫组织化学（IHC）和 Western blot 分析下调软骨组织中 Wnt5a 和 β-catenin 的表达，上调 Sox9 和 Collagen II 的表达，调节 Wnt 信号通路。此外，沉默 Wnt5a 可减少 LPS 诱导的 ADTC5 细胞凋亡，而下调 Wnt5a 与 BGRJ 的联合作用进一步抑制了细胞凋亡。

结论：综上所述，BGRJ 通过 Wnt 信号通路缓解炎症和氧化应激，从而治疗绝经后 KOA。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcab/8513287/9246d61f88fc/12938_2021_939_Fig11_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcab/8513287/c07e25f58d1a/12938_2021_939_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcab/8513287/342eaf68ae2e/12938_2021_939_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcab/8513287/7a3e19cc22fe/12938_2021_939_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcab/8513287/5783305980bb/12938_2021_939_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcab/8513287/24b74e0d9a60/12938_2021_939_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcab/8513287/e279cf6bf251/12938_2021_939_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcab/8513287/15dd88e11678/12938_2021_939_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcab/8513287/2b0d00cfbd44/12938_2021_939_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcab/8513287/07a594510162/12938_2021_939_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcab/8513287/9246d61f88fc/12938_2021_939_Fig11_HTML.jpg

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Exp Mol Med. 2024-2

[2]
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[3]
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[4]
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Ann Transl Med. 2022-9

[5]
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本文引用的文献

[1]
Huoxuezhitong capsule ameliorates MIA-induced osteoarthritis of rats through suppressing PI3K/ Akt/ NF-κB pathway.

Biomed Pharmacother. 2020-9

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J Phys Chem B. 2020-1-23

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Paeoniflorin protects against liver ischemia/reperfusion injury in mice via inhibiting HMGB1-TLR4 signaling pathway.

Phytother Res. 2018-7-26

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