polysaccharide attenuates high-sugar-induced lipid accumulation in HepG2 cells and larvae.

Lipid accumulation is a major factor in the development of non-alcoholic fatty liver disease (NAFLD). Currently, there is a lack of intervention or therapeutic drugs against NAFLD. In this study, we investigated the ability of polysaccharide (SFPS) to reduce lipid accumulation induced by high sugar in HepG2 cells and larvae. The results indicated that SFPS significantly ( < .01) decreased the accumulation of lipid droplets in high sugar-induced HepG2 cells. Furthermore, SFPS also suppressed the expression of and (genes involved in lipogenesis) and increased the expression of and (genes that participated in fatty acid β-oxidation) in these cells. SFPS markedly reduced the content of triglyceride of the third instar larvae developed from eggs reared on the high-sucrose diet. The expression of the and genes in the larvae was also inhibited whereas the expression of two genes involved in the β-oxidation of fatty acids, and was increased in the larval fat body (a functional homolog of the human liver). We also found that SFPS ameliorated developmental abnormalities induced by the high-sucrose diet. These results of this study suggest that SFPS could potentially be used as a therapeutic agent for the prevention and treatment of NAFLD.