来自兔骨骼肌肌球蛋白L-2轻链的胰蛋白酶、糜蛋白酶和胃蛋白酶肽段的氨基酸组成。

The amino acid compositions of the tryptic, chymotryptic and peptic peptides from the L-2 light chain of rabbit skeletal muscle myosin.

作者信息

Matsuda G, Suzuyama Y, Setoguchi M, Maita T, Watanabe B, Maekawa T, Umegane T

出版信息

Hoppe Seylers Z Physiol Chem. 1978 Mar;359(3):357-68.

Abstract

The light chain fraction was separated from rabbit skeletal muscle myosin and four kinds of light chains, L-1, L-2, L-3 and L-4 in the fraction were further isolated by column chromatography using DEAE-cellulose DE-52. After amino-ethylation, the L-2 light chain was digested with trypsin. It was also digested with chymotrypsin and pepsin, respectively, after carboxymethylation. Each of the tryptic, chymotryptic and peptic peptides thus obtained was separated and purified and their amino acid compositions were analyzed.

摘要

从兔骨骼肌肌球蛋白中分离出轻链部分，并用DEAE - 纤维素DE - 52通过柱色谱法进一步分离该部分中的四种轻链L - 1、L - 2、L - 3和L - 4。氨基乙基化后，用胰蛋白酶消化L - 2轻链。羧甲基化后，它也分别用胰凝乳蛋白酶和胃蛋白酶消化。如此获得的每种胰蛋白酶、胰凝乳蛋白酶和胃蛋白酶肽段都进行了分离和纯化，并分析了它们的氨基酸组成。