Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
Faculty of Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran.
Biomed Res Int. 2021 Oct 4;2021:5568113. doi: 10.1155/2021/5568113. eCollection 2021.
MicroRNAs are a large group of small noncoding RNAs that work in multiple cellular pathways. miR-204, as one of the key axes in the development, maintenance, and pathogenesis of the retina, plays several roles by modulating its target genes. This study was aimed at evaluating the target genes of miR-204 involved in the development and progression of common retinopathies such as glaucoma, retinoblastoma, and age-related macular degeneration. In this study, three datasets related to retinopathies (GSE50195, GSE27276, and GSE97508) were selected from Gene Expression Omnibus. miR-204 target genes were isolated from TargeScan. The shares between retinopathy and miR-204 target genes were then categorized. Using Enrichr and STRING, we highlighted the signaling pathways and the relationships between the proteins. SHC1 events in ERBB2, adherent junction's interactions, NGF signaling via TRKA from the plasma membrane, IRF3-mediated activation of type 1 IFN, pathways in upregulated genes and G0 and early G1, RORA-activated gene expression, PERK-regulated gene expression, adherent junction's interactions, and CREB phosphorylation pathways in downregulated genes were identified in glaucoma, retinoblastoma, and age-related macular degeneration. WEE1, SMC2, HMGB1, RRM2, and POLA1 proteins were also observed to be involved in the progression and invasion of retinoblastoma; SLC24A2 and DTX4 in age-related macular degeneration; and EPHB6, EFNB3, and SHC1 in glaucoma. Continuous bioinformatics analysis has shown that miR-204 has a significant presence and expression in retinal tissue, and approximately 293 genes are controlled and regulated by miR-204 in this tissue; also, target genes of miR-204 have the potential to develop various retinopathies; thus, a study of related target genes can provide appropriate treatment strategies in the future.
微小 RNA 是一大组小的非编码 RNA，在多种细胞途径中发挥作用。miR-204 作为视网膜发育、维持和发病机制的关键轴之一，通过调节其靶基因发挥多种作用。本研究旨在评估参与常见视网膜病变（如青光眼、视网膜母细胞瘤和年龄相关性黄斑变性）发展和进展的 miR-204 靶基因。在这项研究中，从基因表达综合数据库中选择了三个与视网膜病变相关的数据集（GSE50195、GSE27276 和 GSE97508）。使用 TargeScan 分离 miR-204 靶基因。然后将视网膜病变和 miR-204 靶基因之间的共享分类。使用 Enrichr 和 STRING，我们突出了信号通路和蛋白质之间的关系。在青光眼、视网膜母细胞瘤和年龄相关性黄斑变性中发现了 ERBB2 中的 SHC1 事件、粘着连接的相互作用、来自质膜的 TRKA 的 NGF 信号、IRF3 介导的 I 型 IFN 的激活、上调基因和 G0 和早期 G1 的途径、RORA 激活基因表达、PERK 调节基因表达、下调基因中的粘着连接的相互作用和 CREB 磷酸化途径。还观察到 WEE1、SMC2、HMGB1、RRM2 和 POLA1 蛋白参与视网膜母细胞瘤的进展和侵袭；SLC24A2 和 DTX4 参与年龄相关性黄斑变性；EPHB6、EFNB3 和 SHC1 参与青光眼。连续的生物信息学分析表明，miR-204 在视网膜组织中具有显著的存在和表达，并且在该组织中大约有 293 个基因受 miR-204 控制和调节；此外，miR-204 的靶基因有可能引发各种视网膜病变；因此，研究相关靶基因可以为未来提供适当的治疗策略。
