Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, China.
Methods Mol Biol. 2022;2368:71-80. doi: 10.1007/978-1-0716-1677-2_5.
Tropisms are growth-based plant directional movements, allowing plants to respond to their living environments. Plant roots have developed various tropic responses, including gravitropism, hydrotropism, chemotropism, and halotropism, in response to the gravity, moisture gradient, nutrient gradient, and salinity gradient, respectively. Revealed mechanisms of several tropic responses suggested that plant hormone gradient and cell division activity play key roles in determining these responses. Approaches to measure cell division and hormone gradients, however, have rarely been applied in root tropic analyses. Here, we describe a number of methods to quantify cell division and hormone gradients during root tropic analysis. These approaches are mainly based on our previous researches on root hydrotropism.
向性是基于生长的植物定向运动，使植物能够对其生活环境做出反应。植物的根已经发展出各种向性反应，包括向重力性、向水性、向化性和向盐性，分别对重力、水分梯度、养分梯度和盐度梯度做出反应。已经揭示的几种向性反应的机制表明，植物激素梯度和细胞分裂活性在决定这些反应中起着关键作用。然而，用于测量细胞分裂和激素梯度的方法在根向性分析中很少被应用。在这里，我们描述了一些在根向性分析中定量细胞分裂和激素梯度的方法。这些方法主要基于我们之前对根向水性的研究。
