Schumann G, Stengel C, Eckardt K, Ihn W
J Basic Microbiol. 1986;26(4):249-55. doi: 10.1002/jobm.3620260416.
Aklanonic acid is an anthracyclinone-related pigment that was found to be an intermediate at an early stage of the biosynthesis of daunomycinone glycosides by Streptomyces griseus. We now isolated anthracycline-negative mutants of other Streptomyces species and used them in feeding experiments with both natural and radioactive aklanonic acid. In cultures of the mutant Streptomyces galilaeus S 727 exogenous aklanonic acid was biotransformed to cinerubin A as the major product. The spectrum of the conversion products was qualitatively comparable to that of the parent strain. In cultures of mutant 21/8 derived from Streptomyces peucetius var. caesius the predominant conversion product was epsilon-rhodomycinone. Daunomycinone glycosides were isolated in small amounts. According to results obtained with the radioactively labelled precursor aklanonic acid was completely incorporated into the molecules of the conversion products. Our findings suggest that aklanonic acid is also a natural intermediate in the biosynthesis of anthracyclines by Streptomyces galilaeus and Streptomyces peucetius. Proposed biosynthetic pathways have been constructed.
阿克拉诺酸是一种与蒽环酮相关的色素，它被发现是灰色链霉菌生物合成柔红霉素酮糖苷早期阶段的一种中间体。我们现在分离出了其他链霉菌属物种的蒽环素阴性突变体，并将它们用于天然和放射性阿克拉诺酸的饲喂实验。在突变型加利利链霉菌S 727的培养物中，外源阿克拉诺酸被生物转化为主要产物灰黄霉素A。转化产物的谱图在质量上与亲本菌株的谱图相当。在源自尖孢链霉菌变种卡氏链霉菌的突变体21/8的培养物中，主要的转化产物是ε-红霉酮。少量分离出了柔红霉素酮糖苷。根据用放射性标记前体获得的结果，阿克拉诺酸完全掺入了转化产物的分子中。我们的发现表明，阿克拉诺酸也是加利利链霉菌和尖孢链霉菌生物合成蒽环素的天然中间体。已经构建了推测的生物合成途径。
