Ma Yuan, Liu Kuichen, Zhang Chunyu, Lin Feng, Hu Wenbo, Jiang Yue, Tao Xianliang, Han Yulin, Han Litao, Liu Chen
College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, Liaoning, China.
Genome. 2021 Oct 14:1-16. doi: 10.1139/gen-2021-0048.
Soybean can provide rich protein and fat and has great economic value worldwide. Cadmium (Cd) is a toxic heavy metal to organisms. It can accumulate in plants and be transmitted to the human body via the food chain. Cd is a serious threat to soybean development, particularly root growth. Some soybean cultivars present tolerant symptoms under Cd stress; however, the potential mechanisms are not fully understood. Here, we optimized RNA-seq to identify the differentially expressed genes (DEGs) in Cd-sensitive (KUAI) and Cd-tolerant (KAIYU) soybean roots and compared the DEGs between KAIYU and KUAI. A total of 1506 and 1870 DEGs were identified in the roots of KUAI and KAIYU, respectively. Through Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, and gene function analyses, we found that genes related to antioxidants and sequestration were responsible for Cd tolerance in KAIYU. In addition, overexpression of , which encodes a heavy metal-transporting ATPase, significantly improved Cd tolerance in transgenic hairy roots. These results provide a preliminary understanding of the tolerance mechanisms in response to Cd stress in soybean root development and are of great importance in developing Cd-resistant soybean cultivars by using the identified DEGs through genetic modification.
大豆能提供丰富的蛋白质和脂肪，在全球具有巨大的经济价值。镉（Cd）是一种对生物体有毒的重金属。它可在植物中积累，并通过食物链传递给人体。镉对大豆发育，尤其是根系生长构成严重威胁。一些大豆品种在镉胁迫下会出现耐受症状；然而，其潜在机制尚未完全明确。在此，我们优化了RNA测序，以鉴定镉敏感型（快）和镉耐受型（开育）大豆根系中的差异表达基因（DEG），并比较了开育和快之间的DEG。在快和开育的根系中分别鉴定出1506个和1870个DEG。通过基因本体论（GO）、京都基因与基因组百科全书（KEGG）途径和基因功能分析，我们发现与抗氧化剂和螯合相关的基因是开育中镉耐受的原因。此外，编码重金属转运ATP酶的基因的过表达显著提高了转基因毛状根中的镉耐受性。这些结果为大豆根系发育中对镉胁迫的耐受机制提供了初步认识，对于通过基因改造利用已鉴定的DEG培育抗镉大豆品种具有重要意义。
