Xie Shan-Rong, Li Yu, Liang Ming-Hua, Yan Bing, Jiang Jian-Guo
School of Food Science and Engineering, South China University of Technology, Guangzhou, 510640, China.
School of Food Science and Engineering, South China University of Technology, Guangzhou, 510640, China.
Enzyme Microb Technol. 2021 Nov;151:109913. doi: 10.1016/j.enzmictec.2021.109913. Epub 2021 Sep 15.
Dunaliella bardawil, a unicellular green alga, can accumulate a large amount of lutein and β-carotene under stresses. Using chemical inducers combined with abiotic stress to promote the accumulation of high value-added products such as lipids and carotenoids in microalgae has attracted more and more attention. In this study, creatinine was added into autotrophic medium to investigate its effects on the growth, chlorophyll content, and the ingredients and content of carotenoids in D. bardawil. The results showed that creatinine alone could significantly increase the biomass, chlorophyll and carotenoid contents of D. bardawil, among which the contents of lutein and β-carotene were further increased, while the content of zeaxanthin was decreased. In order to further improve the content of the two carotenoids, different light intensities combined with creatinine have been adopted. Under 6.589 W/m light intensity, creatinine could effectively increase the production of lutein, zeaxanthin, α-carotene and β-carotene. Compared with the control, the content of lutein increased by 46 % and the content of β-carotene increased by 77 % when the concentration of creatinine was 500 μg/mL. In conclusion, creatinine can effectively improve the production lutein and β-carotene in D. bardawil, which is more conducive under lower light intensity.
巴氏杜氏藻是一种单细胞绿藻，在胁迫条件下能够积累大量叶黄素和β-胡萝卜素。利用化学诱导剂结合非生物胁迫来促进微藻中脂质和类胡萝卜素等高附加值产品的积累已越来越受到关注。在本研究中，将肌酸酐添加到自养培养基中，以研究其对巴氏杜氏藻生长、叶绿素含量以及类胡萝卜素成分和含量的影响。结果表明，单独使用肌酸酐可显著提高巴氏杜氏藻的生物量、叶绿素和类胡萝卜素含量，其中叶黄素和β-胡萝卜素含量进一步增加，而玉米黄质含量降低。为了进一步提高这两种类胡萝卜素的含量，采用了不同光照强度与肌酸酐相结合的方法。在6.589 W/m的光照强度下，肌酸酐可有效提高叶黄素、玉米黄质、α-胡萝卜素和β-胡萝卜素的产量。与对照相比，当肌酸酐浓度为500μg/mL时，叶黄素含量增加了46%，β-胡萝卜素含量增加了77%。总之，肌酸酐能有效提高巴氏杜氏藻中叶黄素和β-胡萝卜素的产量，在较低光照强度下更有利。
