Tang Jie, Li Chaoyi, Zhang Pijun, Lin Wei, Yang Yi
Department of Orthopedics, The Second Affiliated Hospital of Hainan Medical University Haikou 570311, Hainan, China.
Am J Transl Res. 2021 Sep 15;13(9):10670-10675. eCollection 2021.
This study analyzed the predictive value of peripheral blood mononuclear cells' (PBMCs) miR-374a-5p in deep vein thrombosis (DVT) after total hip arthroplasty in the elderly.
There was a total of 112 elderly patients that underwent elective total hip arthroplasty in our hospital and were enrolled as the research subjects. According to the color Doppler ultrasound of lower limbs 5 d postoperatively, subjectswere classified into thrombosis group (n=31) and non-thrombosis group (n=81). We detected the expression levels of miR-374a-5p in PBMCs and plasma D-dimer of the two groups of patients 1 d before surgery, and 1 d, 3 d, and 5 d after surgery, and analyzed the predictive value, and the correlation between miR-374a-5p and plasma D-dimer for DVT by ROC curve.
The relative expression of miR-374a-5p in PBMCs and plasma D-dimer of the two groups 1 d, 3 d, and 5 d after surgery were higher than those on the 1 d before surgery (), and the indexes of thrombus-group-patients were higher than those in the non-thrombosis group at various time points after surgery (). We analyzed the predictive value of miR-374a-5p for DVT by ROC curve, and the areas under the predictive curve of miR-374a-5p on 1 d pre-surgery, and 1 d, 3 d and 5 d post-surgery for DVT were 0.435, 0.820, 0.974 and 0.782 respectively. The areas under the predictive curve of D-dimer for DVT was 0.470, 0.834, 0.863 and 0.790 respectively on 1 d pre-surgery, and 1 d, 3 d and 5 d post-surgery. The correlation analysis indicated that miR-374a-5p was extremely positively correlated with D-dimer ().
miR-374a-5p is of important value in the prediction of DVT in elderly patients with total hip arthroplasty. Its expression level has a critically positive correlation with D-dimer degree, which is likely to be abiologic indicator for the early diagnosis and treatment of DVT.
本研究分析外周血单个核细胞（PBMCs）中miR - 374a - 5p对老年患者全髋关节置换术后深静脉血栓形成（DVT）的预测价值。
选取我院112例行择期全髋关节置换术的老年患者作为研究对象。根据术后5 d下肢彩色多普勒超声检查结果，将患者分为血栓组（n = 31）和非血栓组（n = 81）。检测两组患者术前1 d、术后1 d、3 d和5 d时PBMCs中miR - 374a - 5p的表达水平及血浆D - 二聚体水平，并分析miR - 374a - 5p对DVT的预测价值以及miR - 374a - 5p与血浆D - 二聚体的相关性（采用ROC曲线分析）。
两组患者术后1 d、3 d和5 d时PBMCs中miR - 374a - 5p的相对表达量及血浆D - 二聚体水平均高于术前1 d（），且术后各时间点血栓组患者的各项指标均高于非血栓组（）。采用ROC曲线分析miR - 374a - 5p对DVT的预测价值，术前1 d、术后1 d、3 d和5 d时miR - 374a - 5p对DVT预测曲线下面积分别为0.435、0.820、0.974和0.782。术前1 d、术后1 d、3 d和5 d时D - 二聚体对DVT预测曲线下面积分别为0.470、0.834、0.863和0.790。相关性分析表明，miR - 374a - 5p与D - 二聚体呈极显著正相关（）。
miR - 374a - 5p对老年全髋关节置换术患者DVT的预测具有重要价值，其表达水平与D - 二聚体程度呈显著正相关，有望成为DVT早期诊断及治疗的生物学指标。
