AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan.
Central Blood Institute, Blood Service Headquarters, Japanese Red Cross Society, Tokyo, Japan.
AIDS Res Hum Retroviruses. 2021 Dec;37(12):994-997. doi: 10.1089/AID.2021.0160. Epub 2021 Nov 9.
HIV-1 subtype/circulating recombinant form (CRF) distribution of HIV-1-positive specimens for evaluating HIV diagnostics (IVDs) was examined and compared with the HIV-1 epidemic in Japan. The nucleotide sequences of the gag-pol region of 173 plasma specimens (84, provided in 2007, and 89 in 2013-2015) were determined. HIV-1 subtype/CRF classification was performed based on the phylogenetic analyses of the sequences. The subtype/CRF distribution resulting in this study was similar to that of a previous epidemiological report. Three CRF02_AG and one unique recombinant form, including subtype G and A regions, were observed in the 2013 and 2014 specimens, except in the 2007 specimens. The reference panel consisting of these specimens was practical for the evaluation of HIV IVDs in Japan.
对用于评估 HIV 诊断试剂（IVD）的 HIV-1 阳性标本的 HIV-1 亚型/循环重组形式（CRF）分布进行了检查，并与日本的 HIV-1 流行情况进行了比较。对 173 份血浆标本（84 份于 2007 年提供，89 份于 2013-2015 年提供）的 gag-pol 区核苷酸序列进行了测定。根据序列的系统进化分析进行了 HIV-1 亚型/CRF 分类。本研究得到的亚型/CRF 分布与之前的流行病学报告相似。在 2013 年和 2014 年的标本中观察到 3 种 CRF02_AG 和 1 种独特的重组形式，包括亚型 G 和 A 区，而 2007 年的标本中则没有。由这些标本组成的参考面板对于评估日本的 HIV IVD 是实用的。
